用于增强刚性和原位骨再生的生物相容氧化石墨烯-胶原蛋白复合气凝胶。
Biocompatible graphene oxide-collagen composite aerogel for enhanced stiffness and in situ bone regeneration.
机构信息
Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Department of Plastic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; Department of Pediatrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
出版信息
Mater Sci Eng C Mater Biol Appl. 2019 Dec;105:110137. doi: 10.1016/j.msec.2019.110137. Epub 2019 Aug 27.
The developing bone graft substitutes have become a promising strategy for repairing large bone loss. Aerogels that made from natural polymers were widely investigated for synthetic bone graft due to their high porosity and great biocompatibility. However, the mechanical properties of natural polymer aerogel are extremely poor for large bone repair. Graphene oxide (GO) is one of the nanomaterials with great mechanical properties as well as biocompatibility, making it a promising component when constructing hybrid aerogels for bone regeneration. In the present study, we have developed a highly porous aerogel consist of GO and type I collagen (COL) using sol-gel process (concentrations of GO: 0%, 0.05%, 0.1%, and 0.2% w/v). Results indicated that GO-COL aerogels were highly porous and hydrophilic. Furthermore, the compressive modulus of GO-COL aerogels was enhanced with the GO concentration increased. For in vitro experiment, 0.1% GO-COL aerogel exhibited better biomineralization rate and cell compatibility than other groups of aerogels. For in vivo study, a better bone repair effect was observed in 0.1% GO-COL aerogels than COL aerogel in rat cranial defect models. This study indicated that 0.1% GO-COL aerogel exhibited good biocompatibility and osteogenic ability in vivo, which make it a promising biocompatible scaffold for bone regeneration and tissue engineering.
发展中的骨移植替代物已成为修复大骨缺损的一种有前途的策略。由于具有高孔隙率和良好的生物相容性，由天然聚合物制成的气凝胶被广泛研究用于合成骨移植。然而，天然聚合物气凝胶的机械性能极差，不适合用于大骨修复。氧化石墨烯（GO）是一种具有优异机械性能和生物相容性的纳米材料，在构建用于骨再生的混合气凝胶时，它是一种很有前途的成分。在本研究中，我们使用溶胶-凝胶工艺（GO 浓度：0%、0.05%、0.1%和 0.2%w/v）开发了一种由 GO 和 I 型胶原蛋白（COL）组成的高多孔气凝胶。结果表明，GO-COL 气凝胶具有高度多孔和亲水性。此外，随着 GO 浓度的增加，GO-COL 气凝胶的压缩模量得到增强。在体外实验中，0.1%GO-COL 气凝胶的生物矿化率和细胞相容性优于其他气凝胶组。在体内研究中，在大鼠颅缺损模型中，0.1%GO-COL 气凝胶比 COL 气凝胶表现出更好的骨修复效果。这项研究表明，0.1%GO-COL 气凝胶在体内具有良好的生物相容性和成骨能力，使其成为一种很有前途的骨再生和组织工程生物相容性支架。
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