Beijing Institute of Radiation Medicine, Beijing, 100850, China.
Laboratory of Immunology for Environment and Health, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, Shandong, China.
Arch Pharm Res. 2019 Nov;42(11):1021-1029. doi: 10.1007/s12272-019-01187-0. Epub 2019 Sep 23.
Some flavonoids have been shown to exhibit good antioxidant activity and protect mice from damage induced by radiation. Amentoflavone (AMF), a biflavonoid derived from the traditional herb-Selaginella tamariscina, has been reported to have antioxidant properties. The protective effects and mechanism of action of AMF against radiation injury remain unknown. In this study, male C57BL/6 mice were subjected to total-body Co γ-irradiation at 7.5 or 3.0 Gy. The survival rate and mean survival time were evaluated to determine the radioprotective effect of AMF. Number of peripheral blood cells, frequency of colony forming unit-granulocytes, monocytes and micronuclei were measured to assess the protective effects of AMF on the hematopoietic system. Levels of superoxide dismutase and glutathione, and pathological changes in the bone marrow were determined. Additionally, next-generation sequencing technology was used to explore potential targets of AMF. We observed that AMF markedly extends average survival time, reduces injury to the hematopoietic system and promotes its recovery. Furthermore, treatment with AMF significantly attenuated radiation-induced oxidative stress. In addition, AMF had a significant effect on gene tumor necrosis factor alpha-induced protein 2. Together, the results of this study suggest that AMF is a potential protective agent against radiation injury.
一些类黄酮已被证明具有良好的抗氧化活性，可以保护小鼠免受辐射损伤。穗花杉双黄酮（AMF）是一种从传统草药翠云草中提取的双黄酮，具有抗氧化特性。AMF 对辐射损伤的保护作用及其作用机制尚不清楚。在这项研究中，雄性 C57BL/6 小鼠接受全身 Co γ 射线照射，剂量分别为 7.5Gy 和 3.0Gy。通过评估存活率和平均存活时间来确定 AMF 的辐射防护作用。通过测量外周血血细胞计数、集落形成单位-粒细胞、单核细胞和微核的频率来评估 AMF 对造血系统的保护作用。测定超氧化物歧化酶和谷胱甘肽的水平以及骨髓的病理变化。此外，还使用下一代测序技术来探讨 AMF 的潜在靶点。我们观察到 AMF 显著延长平均存活时间，减轻造血系统损伤并促进其恢复。此外，AMF 治疗可显著减轻辐射诱导的氧化应激。此外，AMF 对肿瘤坏死因子-α诱导蛋白 2 基因有显著影响。总之，这项研究的结果表明 AMF 是一种潜在的辐射损伤防护剂。
