Hochstrasser A C, James R W, Martin B M, Harrington M, Hochstrasser D, Pometta D, Merril C R
Biochemical Genetics Section, National Institute of Mental Health, Bethesda, Maryland 20892.
Appl Theor Electrophor. 1988;1(1):73-6.
The proteins from plasma HDL particles isolated by immunoaffinity chromatography on anti-apolipoprotein A-1 affinity columns have been analysed and purified by high resolution two dimensional gel electrophoresis. Two of the lipoprotein-associated proteins found in the HDL plasma fraction, previously referred to as NA1 and NA2, have also been found in cerebrospinal fluid. After separation by 2DGE, these two proteins were transferred to PVDF membranes, stained and cut out for N-terminal sequencing. The partial sequences (11 and 13 amino acids) obtained for the two HDL particle associated proteins do not match any of those included in the December 1987 National Biomedical Research Foundation (NBRF) database, and there are no significant sequence similarities.
通过抗载脂蛋白A-1亲和柱免疫亲和层析分离的血浆高密度脂蛋白（HDL）颗粒中的蛋白质，已通过高分辨率二维凝胶电泳进行分析和纯化。在HDL血浆组分中发现的两种脂蛋白相关蛋白，以前称为NA1和NA2，也在脑脊液中发现。通过二维凝胶电泳分离后，将这两种蛋白质转移到聚偏二氟乙烯（PVDF）膜上，染色并切下用于N端测序。这两种与HDL颗粒相关的蛋白质获得的部分序列（11和13个氨基酸）与1987年12月国家生物医学研究基金会（NBRF）数据库中包含的任何序列均不匹配，且没有明显的序列相似性。
