INRS-ETE, Université du Québec, Québec, Canada.
J Environ Sci Health B. 2020;55(2):115-126. doi: 10.1080/03601234.2019.1668225. Epub 2019 Sep 24.
The aim of this study was to produce -based biopesticide using starch-producing industry wastewater (SIW) fortified with soybean medium and optimize the formulated product using different adjuvants. This study was necessary as low endotoxin concentration is obtained in formulated biopesticide when SIW alone is used as fermentation medium. The fermentation runs were conducted using SIW alone and SIW fortified with 25% soybean (w/v) medium in 2000 L and 150 L bioreactor, respectively. SIW supplemented with soybean medium showed an increase in cell count (from 1.95 × 10 to 1.65 × 10 CFU mL), spore synthesis (from 1.5 × 10 to 1.35 × 10 CFU mL) and endotoxin concentration (from 436 to 1170 μg mL) when compared to SIW medium alone. The fermented broth was concentrated using continuous centrifugation and adjuvants were added for biopesticide formulation in order to enhance its resistance against UV rays and rainfastness. Entomotoxicity of the formulation produced using fermented broth of SIW fortified with soybean (38,000 IU μL) was higher than that obtained by SIW medium alone (21,000 IU μL), commercial biopesticide Foray 76B (20,000 IU μL) and Btk sander's (12,500 IU μL).
本研究旨在利用富含大豆培养基的淀粉生产工业废水（SIW）生产基于芽孢杆菌的生物农药，并使用不同的助剂对配方产品进行优化。由于单独使用 SIW 作为发酵培养基时，配方生物农药中的内毒素浓度较低，因此进行这项研究是必要的。发酵实验分别在单独使用 SIW 的情况下以及在 2000 L 和 150 L 生物反应器中使用 25%（w/v）大豆培养基强化 SIW 的情况下进行。与单独使用 SIW 相比，大豆培养基强化的 SIW 显示出细胞计数（从 1.95×10 增加到 1.65×10 CFU mL）、孢子合成（从 1.5×10 增加到 1.35×10 CFU mL）和内毒素浓度（从 436 增加到 1170 μg mL）的增加。与单独使用 SIW 相比，大豆培养基强化的 SIW 发酵液经连续离心浓缩后，添加助剂制成生物农药制剂，以增强其对紫外线和耐雨水冲刷的抵抗力。与单独使用 SIW 培养基（38000 IU μL）相比，用大豆强化 SIW 发酵液（38000 IU μL）制成的制剂的杀虫毒性更高，比商业生物农药 Foray 76B（20000 IU μL）和 Btk sander's（12500 IU μL）更高。
