Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot 76100, Israel.
Agricultural Research Organization (ARO), Volcani Center, Institute of Animal Science, HaMaccabim Road, P.O.B 15159, 7528809, Rishon LeTsiyon, Israel.
G3 (Bethesda). 2019 Nov 5;9(11):3867-3875. doi: 10.1534/g3.119.400656.
is one of the most popular ornamental fish species with a higher demand for males due to their large colorful fins. The objectives of this study were mapping of the sex-determining (SD) region on linkage group 12 of guppy, and identification of a sex specific marker. We generated eight polymorphic microsatellite markers distributed along the distal 5.4 Mbp sequence of the previously identified SD region on linkage group (LG) 12. The markers were tested for association with sex in 156 individuals of the Red Blonde and Flame strains, and 126 progeny of four full-sibs Red Blonde families. A male-specific allele was found for microsatellite gu1066 at position of 25.3 Mbp on LG12 for both strains, and gu832 at position 24.4 Mbp for the Flame strain. Thus, a region of 0.9 Mbp between these markers, harboring 27 annotated genes, was selected for analysis. Based on association of copy number variation and sex determination we mapped a duplicated region between LGs 9 and 12, of 1.26 Mbp, containing 59 genes on LG12. The common interval between the segment bounded by gu1066 and gu832, and the duplicated region of 0.43 Mbp on LG12 harbors 11 genes of which 6 have multiple copies (54%). Growth arrest and DNA damage inducible gamma-like (-like) is a plausible positional and functional candidate gene for its role in male fertility. We characterized the genomic structure of the gene in guppy, and found two isoforms; but no sex-biased differences were evident in genomic sequence and gene expression.
是最受欢迎的观赏鱼类之一，由于雄性具有大而彩色的鳍，因此对雄性的需求更高。本研究的目的是对孔雀鱼的第 12 连锁群上的性别决定（SD）区域进行作图，并鉴定一个性别特异性标记。我们在先前确定的第 12 连锁群（LG）SD 区域的远端 5.4 Mbp 序列上生成了 8 个多态微卫星标记。这些标记在 156 个红金和火焰品系个体以及 4 个全同胞红金品系的 126 个后代中进行了与性别相关的测试。在这两个品系中，在 LG12 的 25.3 Mbp 位置发现了微卫星 gu1066 的雄性特异性等位基因，在火焰品系中在 24.4 Mbp 位置发现了 gu832。因此，选择了位于这两个标记之间的 0.9 Mbp 区域进行分析，该区域包含 27 个注释基因。基于拷贝数变异与性别决定的关联，我们在 LG9 和 LG12 之间定位了一个包含 59 个基因的 1.26 Mbp 的重复区域。位于 gu1066 和 gu832 之间的片段与 LG12 上的 0.43 Mbp 重复区域之间的共同区间包含 11 个基因，其中 6 个具有多个拷贝（54%）。生长停滞和 DNA 损伤诱导的 γ 样（γ-like）是一个合理的位置和功能候选基因，因为它在雄性生育力中起作用。我们对孔雀鱼基因的基因组结构进行了表征，发现了两个同工型；但是在基因组序列和基因表达中没有明显的性别偏倚差异。
