Institute of Health and Biomedical Innovation, School of Chemistry, Physics and Mechanical Engineering, Science and Engineering Faculty, Queensland University of Technology, Brisbane QLD 4000, Australia.
Beijing National Laboratory for Molecular Sciences (BNLMS), College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
Nanoscale. 2019 Nov 7;11(41):19497-19505. doi: 10.1039/c9nr04951g. Epub 2019 Sep 25.
Oxidative stress in cells caused by the accumulation of reactive oxygen species (ROS) is a common cause of cell function degeneration, cell death and various diseases. Efficient, robust and inexpensive nanoparticles (nanoenzymes) capable of scavenging/detoxifying ROS even in harsh environments are attracting strong interest. Prussian blue analogues (PBAs), a prominent group of metalorganic nanoparticles (NPs) with the same cyanometalate structure as the traditional and commonly used Prussian blue (PB), have long been envisaged to mimic enzyme activities for ROS scavenging. However, their biological toxicity, especially potential effects on living beings during practical application, has not yet been fully investigated. Here we reveal the enzyme-like activity of FeCo-PBA NPs, and for the first time investigate the effects of FeCo-PBA on cell viability and growth. We elucidate the effect of the nanoenzyme on the ethanol-production efficacy of a typical model organism, the engineered industrial strain Saccharomyces cerevisiae. We further demonstrate that FeCo-PBA NPs have almost no cytotoxicity on the cells over a broad dosage range (0-100 μg mL), while clearly boosting the yeast fermentation efficiency by mitigating oxidative stress. Atmospheric pressure cold plasma (APCP) pretreatment is used as a multifunctional environmental stress produced by the plasma reactive species. While the plasma enhances the cellular uptake of NPs, FeCo-PBA NPs protect the cells from the oxidative stress induced by both the plasma and the fermentation processes. This synergistic effect leads to higher secondary metabolite yields and energy production. Collectively, this study confirms the positive effects of PBA nanoparticles in living cells through ROS scavenging, thus potentially opening new ways to control the cellular machinery in future nano-biotechnology and nano-biomedical applications.
细胞内由活性氧（ROS）积累引起的氧化应激是细胞功能退化、细胞死亡和各种疾病的常见原因。能够在恶劣环境中清除/解毒 ROS 的高效、稳健且廉价的纳米颗粒（纳米酶）正引起强烈关注。普鲁士蓝类似物（PBAs）是一类具有与传统且常用的普鲁士蓝（PB）相同氰合金属结构的金属有机纳米颗粒（NPs），长期以来被设想为模拟酶活性以清除 ROS。然而，其生物毒性，特别是在实际应用中对生物的潜在影响，尚未得到充分研究。在这里，我们揭示了 FeCo-PBA NPs 的类酶活性，并首次研究了 FeCo-PBA 对细胞活力和生长的影响。我们阐明了纳米酶对典型模式生物酿酒酵母乙醇生产功效的影响。我们进一步证明，在较宽的剂量范围内（0-100 μg mL），FeCo-PBA NPs 对细胞几乎没有细胞毒性，同时通过减轻氧化应激显著提高了酵母发酵效率。大气压冷等离子体（APCP）预处理用作由等离子体反应性物质产生的多功能环境应激。虽然等离子体增强了 NPs 的细胞摄取，但 FeCo-PBA NPs 保护细胞免受等离子体和发酵过程引起的氧化应激。这种协同作用导致更高的次生代谢产物产量和能量产生。总的来说，这项研究通过清除 ROS 证实了 PBA 纳米颗粒在活细胞中的积极作用，从而有可能为未来的纳米生物技术和纳米生物医学应用中控制细胞机制开辟新途径。
