Fazleabas A T, Bazer F W, Hansen P J, Geisert R D, Roberts R M
Endocrinology. 1985 Jan;116(1):240-5. doi: 10.1210/endo-116-1-240.
We have compared the distribution of two of the major secreted proteins of the porcine uterus within the endometrium of ovariectomized pigs which had received hormone replacement therapy for 30 days. The proteins studied, plasmin/trypsin inhibitor (PI) and uteroferrin (Uf), an iron-containing acid phosphatase, were both secreted into the uterine lumen by ovariectomized gilts given progesterone (P4) or P4 and 17 beta-estradiol but not by animals given 17 beta-estradiol alone or corn oil. The two proteins were localized immunocytochemically within the endometrium using an immunoperoxidase procedure. The results confirmed that production of PI and Uf was P4-dependent and demonstrated that the primary site of synthesis of PI was the surface and upper glandular epithelium, while Uf synthesis was confined to the glandular epithelium. A similar localization of PI and of Uf was found in endometrial tissue from pigs at day 13 (late luteal phase) of the estrous cycle. These results suggest that the uterine epithelium of the pig is regionally differentiated with regard to the production of P4-induced proteins.
我们比较了接受30天激素替代疗法的去卵巢猪子宫内膜中猪子宫两种主要分泌蛋白的分布情况。所研究的蛋白，即纤溶酶/胰蛋白酶抑制剂（PI）和子宫运铁蛋白（Uf，一种含铁酸性磷酸酶），在接受孕酮（P4）或P4与17β-雌二醇的去卵巢后备母猪中均分泌至子宫腔，但仅接受17β-雌二醇或玉米油的动物则不会分泌。使用免疫过氧化物酶法通过免疫细胞化学将这两种蛋白定位到子宫内膜内。结果证实，PI和Uf的产生依赖于P4，并表明PI的主要合成部位是表面和上层腺上皮，而Uf的合成局限于腺上皮。在发情周期第13天（黄体后期）猪的子宫内膜组织中发现了PI和Uf的类似定位。这些结果表明，猪的子宫上皮在P4诱导蛋白的产生方面存在区域分化。
