Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Laboratory of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Beijing National Laboratory for Molecular Sciences (BNLMS), CAS Key Laboratory of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China; CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing 100190, China.
J Colloid Interface Sci. 2019 Dec 1;557:628-634. doi: 10.1016/j.jcis.2019.09.033. Epub 2019 Sep 11.
Molecular assemblies of cationic dipeptide-gold nanoparticle (CDP-AuNP) hybrid microspheres were used to modify cholesterol oxidase electrodes for high-sensitivity cholesterol detection. The cationic dipeptide used here serves as a functional molecule for adsorbing chloroauric acid based on electrostatic interactions and assembly into spherical structures, providing a platform for loading gold nanoparticles (AuNPs), increasing the immobilization load of the enzyme and maintaining the activity of the enzyme as a result of excellent biocompatibility. Moreover, the CDP-AuNP modified cholesterol oxidase electrode has a higher electrocatalytic activity to cholesterol with obvious enhancement in the current response, exhibiting a current response 13 times higher than that of the controlled electrode. The outstanding biocompatibility and increased enzyme load by hybrid microspheres and the good charge-transfer ability of AuNPs in the peptide-based electrode indicate a very attractive perspective in the field of biodevices.
阳离子二肽-金纳米粒子（CDP-AuNP）杂化微球的分子组装体被用于修饰胆固醇氧化酶电极，以实现高灵敏度胆固醇检测。这里使用的阳离子二肽作为一种基于静电相互作用吸附氯金酸并组装成球形结构的功能分子，为负载金纳米粒子（AuNPs）提供了一个平台，增加了酶的固定化负载，并由于其出色的生物相容性而保持了酶的活性。此外，CDP-AuNP 修饰的胆固醇氧化酶电极对胆固醇具有更高的电催化活性，电流响应明显增强，比对照电极高 13 倍。杂化微球的出色生物相容性和增加的酶负载以及基于肽的电极中 AuNPs 的良好电荷转移能力表明，在生物器件领域具有非常有吸引力的前景。
