Teillaud J L, Diamond B, Pollock R R, Fajtova V, Scharff M D
J Immunol. 1985 Mar;134(3):1774-9.
The specificity of the Fc gamma receptors on the X63.Ag8.653 nonproducing myeloma cell line has been examined for binding to IgG1-, IgG2a-, and IgG2b-containing antigen-antibody complexes. Complexes containing each of these subclasses bind, and the binding of each is inhibited by the others. Trypsin treatment did not inhibit the binding of any of these subclasses. Furthermore, the monoclonal anti-Fc receptor antibody 2.4G2 inhibits the binding of all three subclasses. These results, together with those of other investigators, suggest that there is a single FcR for IgG1, IgG2a, and IgG2b on mouse B cells which differs in its specificity from the macrophage Fc gamma R. This is confirmed by the fact that a mutant IgG2b myeloma protein which binds to the macrophage Fc gamma 1/gamma 2b receptor does not bind to the Fc gamma R on X63.Ag8.653.
已对X63.Ag8.653非分泌型骨髓瘤细胞系上Fcγ受体与含IgG1、IgG2a和IgG2b的抗原-抗体复合物结合的特异性进行了检测。含有这些亚类中每一种的复合物均可结合，且每一种的结合都可被其他亚类所抑制。胰蛋白酶处理并未抑制这些亚类中任何一种的结合。此外，单克隆抗Fc受体抗体2.4G2可抑制所有这三种亚类的结合。这些结果，连同其他研究者的结果，提示在小鼠B细胞上存在一种针对IgG1、IgG2a和IgG2b的单一FcR，其特异性不同于巨噬细胞FcγR。这一点已被以下事实所证实：一种与巨噬细胞Fcγ1/γ2b受体结合的突变型IgG2b骨髓瘤蛋白并不与X63.Ag8.653上的FcγR结合。
