ZBSA - Center for Biological Systems Analysis, Albert-Ludwigs-University Freiburg, Habsburgerstrasse. 49, D-79104, Freiburg, Germany.
Faculty for Biology, Albert-Ludwigs-University Freiburg, Schaenzlestrasse 1, D-79104, Freiburg, Germany.
Sci Rep. 2019 Sep 26;9(1):13940. doi: 10.1038/s41598-019-50371-1.
Analogous to a photocopier, we developed a DNA microarray copy technique and were able to copy patterned original DNA microarrays. With this process the appearance of the copied DNA microarray can also be altered compared to the original by producing copies of different resolutions. As a homage to the very first photocopy made by Chester Charlson and Otto Kornei, we performed a lookalike DNA microarray copy exactly 80 years later. Those copies were also used for label-free real-time kinetic binding assays of apo-dCas9 to double stranded DNA and of thrombin to single stranded DNA. Since each DNA microarray copy was made with only 5 µl of spPCR mix, the whole process is cost-efficient. Hence, our DNA microarray copier has a great potential for becoming a standard lab tool.
类似于复印机，我们开发了一种 DNA 微阵列复制技术，能够复制有图案的原始 DNA 微阵列。通过这个过程，与原始微阵列相比，我们还可以通过产生不同分辨率的复制品来改变复制微阵列的外观。为了向 Chester Charlson 和 Otto Kornei 制作的第一张影印件致敬，我们在整整 80 年后精确地复制了一份外观相似的 DNA 微阵列。这些复制品还用于无标记的实时动力学 apo-dCas9 与双链 DNA 以及凝血酶与单链 DNA 的结合分析。由于每个 DNA 微阵列的复制仅使用了 5 μl 的 spPCR 混合物，因此整个过程具有成本效益。因此，我们的 DNA 微阵列复印机具有成为标准实验室工具的巨大潜力。
