Pan Leilei, Jiang Yanan, Zhou Wenjie, Jiang Pingping, Wu Lanrong, Chen Ao, Zhu Hong, Sui Jiongming, Wang Jingshan, Qiao Lixian
Dry-land Farming Technology Laboratory, College of Agronomy, Qingdao Agricultural University, Qingdao 266109, Shandong, China.
Key Laboratory of Plant Biotechnology in Universities of Shandong Province, College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, Shandong, China.
Sheng Wu Gong Cheng Xue Bao. 2019 Sep 25;35(9):1698-1706. doi: 10.13345/j.cjb.190045.
Yuhua91 is a new peanut variety with high oleic acid content bred by Qingdao Agricultural University. The crossing was conducted with Luhua11 as female parent and with Kainong1715, an F435-type variety with high oleic acid content as male parent. The real F1 hybrids were screened by sequencing on PCR amplification products, and those homozygotes with bb genotype in F2 populations were screened by the same sequencing method as above. The content of oleic and linoleic acid was measured on the kernels harvested from F2 single plants by near infrared ray method, and those kernels whose content of oleic was above 80%, oleic and linoleic acid ratio was above 10.0 were obtained and planted into a row, with pedigree method for subsequent selection breeding. Yuhua91 has some characters of small pod, light and obvious pod texture, 148.06 g per 100 pods, 63.31 g per 100 kernels, 75.15% shelling percentage, long elliptic seed kernel, pink seed coat, without crack, white endotesta. Its content of protein, oil, oleic acid, linoleic acid and palmitic acid was 26.57%, 52.72%, 80.40%, 2.50% and 5.57% respectively. Yuhua91 has other characters of strong seedlings, compact pod areas, and moderate resistance to leaf spot disease and bacterial wilt. Average pod yield is 215.79 kg per Mu, 15.27% higher than the control variety Huayu20. Average seed kernels yield is 157.33 kg per Mu, 21.64% higher than the control variety Huayu20. Yuhua 91 has been registered on department of agriculture in 2018, and the registration No. is GPD peanut (2018) 370210, fit for growing in Shandong Province.
豫花91是青岛农业大学培育的高油酸含量花生新品种。以鲁花11为母本,以高油酸含量的F435类型品种开农1715为父本进行杂交。通过对PCR扩增产物进行测序筛选出真正的F1杂种,并采用上述相同的测序方法在F2群体中筛选出bb基因型的纯合子。采用近红外法测定F2单株收获籽粒的油酸和亚油酸含量,获得油酸含量在80%以上、油酸与亚油酸比值在10.0以上的籽粒,单行种植,采用系谱法进行后续选择育种。豫花91具有荚果小、荚果质地轻且明显、百果重148.06克、百仁重63.31克、出仁率75.15%、籽仁长椭圆形、种皮粉红色、无裂纹、内种皮白色等特征。其蛋白质、脂肪、油酸、亚油酸和棕榈酸含量分别为26.57%、52.72%、80.40%、2.50%和5.57%。豫花91还具有幼苗健壮、荚果区紧凑、抗叶斑病和青枯病能力中等的特点。平均荚果产量为每亩215.79千克,比对照品种花育20增产15.27%。平均籽仁产量为每亩157.33千克,比对照品种花育20增产21.64%。豫花91于2018年通过农业部门登记,登记编号为GPD花生(2018)370210,适宜在山东省种植。