Tang Yanyan, Qiu Xiaochen, Hu Changli, Li Jingjing, Wu Lanrong, Wang Weihua, Li Xin, Li Xiaoting, Zhu Hong, Sui Jiongming, Wang Jingshan, Qiao Lixian
College of Agronomy, Qingdao Agricultural University, Dry-Land Farming Technology Laboratory of Shandong Province, Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application, Qingdao, 266109 China.
Qingdao Seed Station, Qingdao, 266071 China.
Mol Breed. 2022 Jul 14;42(7):42. doi: 10.1007/s11032-022-01313-9. eCollection 2022 Jul.
Peanut ( L.) is an important crop used for oil production, and oleic acid is a major factor in determining oil quality. Alterations in the oleic acid content can improve the nutritional quality and oxidative stability and prolong the shelf life of peanut products. The objective of this study was to develop a peanut variety with a high-oleic-acid content and high yield. One elite variety, "huayu22," was hybridized with the high-oleic-acid "KN176" donor and backcrossed for four generations as the recurrent parent using marker-assisted backcross selection. Based on the Kompetitive allele-specific PCR (KASP) screening of markers, the oleic acid content of advanced generations derived by selfing was assessed by near-infrared reflectance spectroscopy and gas chromatography. The genetic background recovery rate of four BCF lines showed an average of 92.34% and was confirmed by genotyping using the Axiom_ 58 K SNP array. Across these superior lines in BCF generations, one line with a high-oleic-acid content and high yield was detected and named "YH61." In particular, yield comparison experiments showed that YH61 exhibited high and stable yield at three different locations and was moderately resistant to leaf spot disease. The distinctness, uniformity and stability (DUS) testing for two consecutive years suggested that YH61 reached the standard for variety rights application. The use of the peanut variety YH61 contributed to the expansion of the cultivation area due to its high value in the oleic acid market and the proven economic benefits in China. This study demonstrated that the marker-assisted backcross strategy based on a cost-effective KASP assay and SNP array for the detection of mutations in and genetic background evaluation can be used to create efficient peanut breeding programs and contribute to oil quality and high-yield stability.
The online version contains supplementary material available at 10.1007/s11032-022-01313-9.
花生（Arachis hypogaea L.）是一种用于生产食用油的重要作物，油酸是决定油品质的主要因素。油酸含量的改变可以改善营养品质和氧化稳定性，并延长花生产品的货架期。本研究的目的是培育出高油酸含量且高产的花生品种。一个优良品种“花育22号”与高油酸“KN176”供体杂交，并以其为轮回亲本进行了四代回交，采用标记辅助回交选择。基于竞争性等位基因特异性PCR（KASP）标记筛选，通过近红外反射光谱法和气相色谱法对自交后代的油酸含量进行了评估。四个回交一代（BCF）株系的遗传背景恢复率平均为92.34%，并使用Axiom_58K SNP芯片进行基因分型确认。在这些BCF世代的优良株系中，检测到一个高油酸含量且高产的株系，并命名为“YH61”。特别是产量比较试验表明，YH61在三个不同地点均表现出高产且稳定，对叶斑病具有中度抗性。连续两年的特异性、一致性和稳定性（DUS）测试表明，YH61达到了品种权申请标准。花生品种YH61因其在油酸市场的高价值以及在中国已证实的经济效益，有助于扩大种植面积。本研究表明，基于具有成本效益的KASP检测和SNP芯片用于检测突变和遗传背景评估的标记辅助回交策略，可用于创建高效的花生育种计划，并有助于提高油品质和高产稳定性。
在线版本包含可在10.1007/s11032-022-01313-9获取的补充材料。
