Asano Mamiko, Tanaka Satoshi, Sakaguchi Minoru
Department of pharmacy, Osaka University of Pharmaceutical Sciences, Osaka, Japan.
Biosci Biotechnol Biochem. 2020 Jan;84(1):103-110. doi: 10.1080/09168451.2019.1670044. Epub 2019 Sep 27.
We previously reported that MDA-MB-231 and MCF-7 cells, which are breast cancer cell lines and have cancer and cancer-initiating cells (CICs), were killed following normothermic microwave irradiation in which the cellular temperature was maintained at 37°C. In this study, we investigated the percentages of live or dead cells among CD44/CD24 cells, which were defined as CICs among MDA-MB-231 and MCF-7 cells, and other types of cells in response to microwave irradiation. CD44/CD24 cells among MDA-MB-231 cells were killed, thereby decreasing the number of cells, whereas the number of live CD44/CD24 MCF-7 cells was increased following microwave irradiation. Moreover, adhesion, invasion, and migration were decreased in MDA-MB-231 cells, and the activation of matrix metalloproteinase-2 (MMP-2) in MDA-MB-231 cells was increased following microwave irradiation. These decreased cell activities might have been caused by MMP-2 activation and population changes in CD44/CD24 in MDA-MB-231 cells. APC: allophecocyanin; CBB: coomassie Brilliant Blue; CD: cluster of differentiation; CICs: cancer-initiating cells; FACS: fluorescence-activated cell sorting; FBS: fetal bovine serum; FITC: fluorescein isothiocyanate; FTDT: finite-difference time domain; HER2: human epidermal growth factor receptor type 2; PI: propidium iodide.
我们之前报道过，MDA-MB-231和MCF-7细胞（均为乳腺癌细胞系，含有癌细胞和癌症起始细胞（CICs））在细胞温度维持在37°C的常温微波照射后会被杀死。在本研究中，我们调查了在MDA-MB-231和MCF-7细胞中被定义为CICs的CD44/CD24细胞以及其他类型细胞中活细胞或死细胞的百分比，以响应微波照射。MDA-MB-231细胞中的CD44/CD24细胞被杀死，从而使细胞数量减少，而微波照射后MCF-7活CD44/CD24细胞的数量增加。此外，MDA-MB-231细胞的黏附、侵袭和迁移能力下降，微波照射后MDA-MB-231细胞中基质金属蛋白酶-2（MMP-2）的活性增加。这些细胞活性的下降可能是由MDA-MB-231细胞中MMP-2的激活以及CD44/CD24的群体变化引起的。APC：别藻青蛋白；CBB：考马斯亮蓝；CD：分化簇；CICs：癌症起始细胞；FACS：荧光激活细胞分选；FBS：胎牛血清；FITC：异硫氰酸荧光素；FTDT：时域有限差分法；HER2：人表皮生长因子受体2型；PI：碘化丙啶。
