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蜱传脑炎病毒抑制人类神经来源细胞中的 rRNA 合成和宿主蛋白产生。

Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin.

机构信息

Institute of Parasitology, Biology Centre of the Czech Academy of Sciences, Branišovská 31, České Budějovice, Czech Republic.

Faculty of Science, University of South Bohemia in České Budějovice, Branišovská, České Budějovice, Czech Republic.

出版信息

PLoS Negl Trop Dis. 2019 Sep 27;13(9):e0007745. doi: 10.1371/journal.pntd.0007745. eCollection 2019 Sep.

DOI:10.1371/journal.pntd.0007745
PMID:31560682
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6785130/
Abstract

Tick-borne encephalitis virus (TBEV), a member of the genus Flavivirus (Flaviviridae), is a causative agent of a severe neuroinfection. Recently, several flaviviruses have been shown to interact with host protein synthesis. In order to determine whether TBEV interacts with this host process in its natural target cells, we analysed de novo protein synthesis in a human cell line derived from cerebellar medulloblastoma (DAOY HTB-186). We observed a significant decrease in the rate of host protein synthesis, including the housekeeping genes HPRT1 and GAPDH and the known interferon-stimulated gene viperin. In addition, TBEV infection resulted in a specific decrease of RNA polymerase I (POLR1) transcripts, 18S and 28S rRNAs and their precursor, 45-47S pre-rRNA, but had no effect on the POLR3 transcribed 5S rRNA levels. To our knowledge, this is the first report of flavivirus-induced decrease of specifically POLR1 rRNA transcripts accompanied by host translational shut-off.

摘要

蜱传脑炎病毒（TBEV）是黄病毒属（黄病毒科）的一员，是一种严重的神经感染病原体。最近，一些黄病毒已被证明与宿主蛋白合成相互作用。为了确定 TBEV 是否在其自然靶细胞中与该宿主过程相互作用，我们分析了源自小脑髓母细胞瘤的人细胞系（DAOY HTB-186）中的从头蛋白合成。我们观察到宿主蛋白合成率显著下降，包括管家基因 HPRT1 和 GAPDH 以及已知的干扰素刺激基因 viperin。此外，TBEV 感染导致 RNA 聚合酶 I（POLR1）转录物、18S 和 28S rRNA 及其前体 45-47S 前 rRNA 的特异性减少，但对 POLR3 转录的 5S rRNA 水平没有影响。据我们所知，这是首次报道黄病毒诱导的特异性 POLR1 rRNA 转录物减少伴随着宿主翻译关闭。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1371/6785130/47caa5baafd9/pntd.0007745.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1371/6785130/e81fa4472ca2/pntd.0007745.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1371/6785130/8bccce95e3a8/pntd.0007745.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1371/6785130/2f9595b8f5cb/pntd.0007745.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1371/6785130/a450ae692282/pntd.0007745.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1371/6785130/4ff4263e54c9/pntd.0007745.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1371/6785130/1de6fe2cd15b/pntd.0007745.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1371/6785130/d2361a1437ce/pntd.0007745.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1371/6785130/47caa5baafd9/pntd.0007745.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1371/6785130/e81fa4472ca2/pntd.0007745.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1371/6785130/8bccce95e3a8/pntd.0007745.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1371/6785130/2f9595b8f5cb/pntd.0007745.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1371/6785130/a450ae692282/pntd.0007745.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1371/6785130/4ff4263e54c9/pntd.0007745.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1371/6785130/1de6fe2cd15b/pntd.0007745.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1371/6785130/d2361a1437ce/pntd.0007745.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1371/6785130/47caa5baafd9/pntd.0007745.g008.jpg

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