College of Sciences, China Agricultural University, No. 2 Yuan mingyuan West Road, Haidian District, Beijing, 100193, China.
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China.
Chemosphere. 2020 Feb;240:124900. doi: 10.1016/j.chemosphere.2019.124900. Epub 2019 Sep 18.
Spirotetramat (SPT) is a new tetronic acid derivative insecticide used to control scales and aphids; the potential for endocrine disruptor effects in fish could not be finalized with the available data. In this study, zebrafish were selected to assess the endocrine-disrupting effects. Significant decrease of plasma estradiol (E2), testosterone (T) and 11-ketotestosterone (11-KT) were observed in both male and female following the spirotetramat exposure; the vitellogenin (VTG) level in females significantly decreased. The expression of the hypothalamic-pituitary-gonad (HPG) axis genes fshr, lhr and esr1 showed significant increase in the gonads, which expression in males is higher than in females. In addition, the activities of capspase-3 and caspase-9 significantly decreased in both males and females liver, while the capspase-3 and caspase-9 were increased in male testis, the mRNA expression levels of genes expression related to the apoptosis pathway were also significantly altered after the spirotetramat exposure. Additionally, we found the parental zebrafish exposed to spirotetramat induced the development delay of its offspring. Above all, the adverse effects induced by spirotetramat suggesting that spirotetramat is a potential exogenous hazardous agent.
螺虫乙酯（SPT）是一种新型四氢呋喃酸衍生物杀虫剂，用于防治介壳虫和蚜虫；但现有数据无法确定其对鱼类是否具有内分泌干扰作用。在这项研究中，选择斑马鱼来评估其内分泌干扰作用。螺虫乙酯暴露后，雌雄鱼的血浆雌二醇（E2）、睾酮（T）和 11-酮睾酮（11-KT）水平均显著下降；雌鱼的卵黄蛋白原（VTG）水平显著下降。下丘脑-垂体-性腺（HPG）轴基因 fshr、lhr 和 esr1 在性腺中的表达显著增加，而在雄性中的表达高于雌性。此外，雌雄鱼肝脏中的 capspase-3 和 caspase-9 活性均显著下降，而雄性睾丸中的 capspase-3 和 caspase-9 活性增加，螺虫乙酯暴露后与细胞凋亡途径相关的基因表达水平也发生了显著改变。此外，我们发现暴露于螺虫乙酯的亲鱼会导致其后代发育迟缓。综上所述，螺虫乙酯引起的不良影响表明其是一种潜在的外源性有害因素。
