Włodarczyk Agnieszka, Wilczek Grażyna, Wilczek Piotr, Student Sebastian, Ostróżka Anna, Tarnawska Monika, Rost-Roszkowska Magdalena
Department of Animal Histology and Embryology, University of Silesia in Katowice, Katowice, Poland.
Department of Animal Physiology and Ecotoxicology, University of Silesia in Katowice, Katowice, Poland.
PeerJ. 2019 Sep 11;7:e7399. doi: 10.7717/peerj.7399. eCollection 2019.
The middle region of the digestive system, the midgut of freshwater shrimp is composed of a tube-shaped intestine and the hepatopancreas formed by numerous caeca. Two types of cells have been distinguished in the intestine, the digestive cells (D-cells) and regenerative cells (R-cells). The hepatopancreatic tubules have three distinct zones distinguished along the length of each tubule-the distal zone with R-cells, the medial zone with differentiating cells, and the proximal zone with F-cells (fibrillar cells) and B-cells (storage cells). Fasting causes activation of cell death, a reduction in the amount of reserve material, and changes in the mitochondrial membrane potential. However, here we present how the concentration of ROS changes according to different periods of fasting and whether re-feeding causes their decrease. In addition, the activation/deactivation of mitochondrial superoxide dismutase (MnSOD) was analyzed. The freshwater shrimps (Crustacea, Malacostraca, Decapoda) were divided into experimental groups: animals starved for 14 days, animals re-fed for 4, 7, and 14 days. The material was examined using the confocal microscope and the flow cytometry. Our studies have shown that long-term starvation increases the concentration of free radicals and MnSOD concentration in the intestine and hepatopancreas, while return to feeding causes their decrease in both organs examined. Therefore, we concluded that a distinct relationship between MnSOD concentration, ROS activation, cell death activation and changes in the mitochondrial membrane potential occurred.
消化系统的中间区域，即淡水虾的中肠，由管状肠道和众多盲囊形成的肝胰腺组成。在肠道中已区分出两种类型的细胞，即消化细胞（D细胞）和再生细胞（R细胞）。肝胰腺小管沿每个小管的长度有三个不同的区域——带有R细胞的远端区域、带有分化细胞的中间区域以及带有F细胞（纤维状细胞）和B细胞（储存细胞）的近端区域。禁食会导致细胞死亡激活、储备物质减少以及线粒体膜电位变化。然而，在这里我们展示了活性氧（ROS）浓度如何根据禁食的不同时期而变化，以及重新喂食是否会导致其浓度降低。此外，还分析了线粒体超氧化物歧化酶（MnSOD）的激活/失活情况。淡水虾（甲壳纲，软甲亚纲，十足目）被分为实验组：饥饿14天的动物、重新喂食4天、7天和14天的动物。使用共聚焦显微镜和流式细胞仪对材料进行了检查。我们的研究表明，长期饥饿会增加肠道和肝胰腺中自由基的浓度以及MnSOD的浓度，而恢复喂食会导致在所检查的两个器官中其浓度降低。因此，我们得出结论，MnSOD浓度、ROS激活、细胞死亡激活以及线粒体膜电位变化之间存在明显的关系。
