University of South Bohemia, Faculty of Sciences, Department of Molecular Biology and Genetics, Branišovska 1160/31, 37005 České Budějovice, Czech Republic and University of Chemistry and Technology Prague, Department of Chemical Engineering, Technická 3, 166 28 Prague, Czech Republic.
University of Chemistry and Technology Prague, Department of Chemical Engineering, Technická 3, 166 28 Prague, Czech Republic.
Biomater Sci. 2019 Nov 1;7(11):4708-4719. doi: 10.1039/c9bm00539k. Epub 2019 Sep 30.
Glucan particles derived from yeast have been recently proposed as potential drug delivery carriers. Here, we demonstrate the potential of glucan particles for protein delivery in vivo, using the insect Drosophila melanogaster as a model organism. By employing genetic tools, we demonstrate the capacity of yeast glucan particles to spread efficiently through the Drosophila body, to enter macrophages and to deliver an active transcription factor protein successfully. Moreover, the glucan particles were nontoxic and induced only minimal immune response. The injection of glucan particles did not impair the ability of Drosophila to fight and survive infection by pathogenic bacteria. From this study, Drosophila emerges as an excellent model to test and develop drug delivery systems based on glucan particles, specifically aimed to regulate macrophages.
最近，有人提出从酵母中提取的葡聚糖颗粒可作为潜在的药物输送载体。在这里，我们以昆虫果蝇为模型生物，证明了葡聚糖颗粒在体内输送蛋白质的潜力。通过遗传工具，我们证明了酵母葡聚糖颗粒能够有效地在果蝇体内传播，进入巨噬细胞并成功输送活性转录因子蛋白。此外，葡聚糖颗粒没有毒性，只引起最小的免疫反应。葡聚糖颗粒的注射不会损害果蝇抵御和抵抗致病性细菌感染的能力。这项研究表明，果蝇是测试和开发基于葡聚糖颗粒的药物输送系统的极佳模型，特别是旨在调节巨噬细胞的系统。
