Phelps Mandy S, Verbeck Guido F
University of North Texas, Denton, TX, USA.
Methods Mol Biol. 2020;2064:19-30. doi: 10.1007/978-1-4939-9831-9_3.
The ability to discriminately analyze the chemical constituents of single cells and organelles is highly sought after and necessary to establish true biomarkers. Some major challenges of individual cell analysis include requirement and expenditure of a large sample of cells as well as extensive extraction and separation techniques. Here, we describe methods to perform individual cell and organelle extractions of both tissues and cells in vitro using nanomanipulation coupled to mass spectrometry. Lipid profiles display heterogeneity from extracted adipocytes and lipid droplets, demonstrating the necessity for single cell analysis. The application of these techniques can be applied to other cell and organelle types for selective and thorough monitoring of disease progression and biomarker discovery.
能够对单个细胞和细胞器的化学成分进行鉴别分析，是建立真正生物标志物的迫切需求且必不可少的。单个细胞分析的一些主要挑战包括需要大量细胞样本及其耗费，以及广泛的提取和分离技术。在此，我们描述了使用与质谱联用的纳米操作在体外对组织和细胞进行单个细胞和细胞器提取的方法。脂质谱显示出从提取的脂肪细胞和脂滴中呈现出的异质性，这证明了单细胞分析的必要性。这些技术的应用可扩展到其他细胞和细胞器类型，用于对疾病进展进行选择性和全面的监测以及生物标志物的发现。
