Romero P A, Friedlander P, Herscovics A
FEBS Lett. 1985 Apr 8;183(1):29-32. doi: 10.1016/0014-5793(85)80947-9.
The lipid-linked oligosaccharides synthesized in the presence of the alpha-glucosidase inhibitors, 1-deoxynojirimycin (DJN) and N-methyl-1-deoxynojirimycin (MDJN), were compared in IEC-6 intestinal epithelial cells in culture. HPLC analysis of the oligosaccharides obtained before and after exhaustive jack bean alpha-mannosidase digestion indicates that control and MDJN-treated cells synthesize similar amounts of Glc3Man9GlcNAc2-PP-dolichol. In contrast, the formation of this compound is greatly reduced in DJN-treated cells, the major lipid-linked oligosaccharide found being Man9GlcNAc2-PP-dolichol. The decreased availability of the preferred donor for protein glycosylation may account for the impaired glycosylation and secretion of certain glycoproteins in the presence of DJN.
在培养的IEC-6肠上皮细胞中，对在α-葡萄糖苷酶抑制剂1-脱氧野尻霉素（DJN）和N-甲基-1-脱氧野尻霉素（MDJN）存在下合成的脂质连接寡糖进行了比较。对经彻底的刀豆α-甘露糖苷酶消化前后获得的寡糖进行HPLC分析表明，对照细胞和经MDJN处理的细胞合成的Glc3Man9GlcNAc2-PP-多萜醇量相似。相比之下，在经DJN处理的细胞中，这种化合物的形成大大减少，发现的主要脂质连接寡糖是Man9GlcNAc2-PP-多萜醇。在DJN存在的情况下，蛋白质糖基化的首选供体可用性降低可能是某些糖蛋白糖基化和分泌受损的原因。
