DSM-Department of Medical Sciences-University of Trieste, 34149 Trieste, Italy.
Doctorate of Nanotechnology-University of Trieste, 34100 Trieste, Italy.
Int J Mol Sci. 2019 Sep 27;20(19):4819. doi: 10.3390/ijms20194819.
In clinical practice, patients' tissues are fixed and paraffin-embedded in order to enable histological diagnosis. Nowadays, those tissues are also used for molecular characterization. Formalin is the most used fixative worldwide, and Bouin's solution in some worldwide institutions. Among molecular targets, micro RNAs (miRNAs), the single-stranded non-coding RNAs comprised of 18 to 24 nucleotides, have been demonstrated to be resistant to fixation and paraffin-embedding processes, with consequent possible application in clinical practice. In the present study, , , , , , , and were investigated in formalin and matched Bouin's solution-fixed tissues of high grade serous ovarian cancers by means of real-time and droplet digital PCR (ddPCR). Micro RNAs were detectable and analyzable in both formalin- and Bouin's-fixed specimens, but on average, higher Ct values and lower copies/µL were found in Bouin's-fixed samples. Data from formalin-fixed samples correlated significantly for most targets with Bouin's ones, except for and . This study shows that miRNAs are analyzable in both formalin- and Bouin's-fixed specimens, with the possibility, after proper data normalization, to compare miRNA-based data from formalin-fixed samples to those of Bouin's-fixed ones.
在临床实践中，为了进行组织学诊断，患者的组织被固定和石蜡包埋。如今，这些组织也被用于分子特征分析。福尔马林是世界范围内使用最广泛的固定剂，而在一些世界范围内的机构中使用的是Bouin 溶液。在分子靶标中，微 RNA（miRNA）是由 18 到 24 个核苷酸组成的单链非编码 RNA，已被证明能抵抗固定和石蜡包埋过程，因此可能在临床实践中有应用。在本研究中，采用实时和液滴数字 PCR（ddPCR）方法，研究了高级别浆液性卵巢癌中福尔马林和匹配的 Bouin 溶液固定组织中的 、 、 、 、 、和 。miRNA 可在福尔马林和 Bouin 固定标本中检测和分析，但平均而言，Bouin 固定标本的 Ct 值更高，拷贝数/µL 更低。福尔马林固定样本的数据与 Bouin 样本的大多数靶标都有显著相关性，除了 和 。本研究表明，miRNA 可在福尔马林和 Bouin 固定标本中进行分析，在适当的数据归一化后，有可能将福尔马林固定样本的 miRNA 数据与 Bouin 固定样本的数据进行比较。
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