Lippiello L, Kaye C, Neumata T, Mankin H J
Connect Tissue Res. 1985;13(2):99-107. doi: 10.3109/03008208509152388.
Bovine and human articular cartilage segments exhibit variable alterations in metabolism following in vitro exposure to hydrostatic pressure. A decrease in incorporation of the labeled substrates 35SO4, 3H-glycine and 3H-uridine to values less than 50% of non-pressurized tissue results from exposure to pressures between 75 and 300 psi. A pressure of 375 psi consistently results in a 10-15% increase in cartilage synthetic activity in the presence or absence of 10% fetal calf serum. Dialyzed fetal calf serum increases the metabolic response at 375 psi from 10% to 55%. The recovery phase following exposure to pressure includes a release (rebound) phenomenon whereby a burst of metabolic activity elevates the metabolic rate to normal levels when the tissues are inhibited (75-300 psi) and accelerates the metabolic rate by 60% in tissues whose metabolism was elevated (375 psi). These data suggest that articular cartilage chondrocytes have the capacity to rapidly and differentially transform mechanical signals derived from application of hydrostatic pressure into metabolic events. The direction of the response is apparently dictated by the magnitude of the applied force and presence of dialyzable components in serum. Although the force applied only partially mimics in vivo forces, the observed responses to pressure support the thesis that pressure modulation of metabolic activity in articular cartilage may be an important factor in its maintenance.
牛和人的关节软骨段在体外暴露于静水压力后，其代谢会出现不同程度的改变。暴露于75至300磅力/平方英寸的压力下，标记底物35SO4、3H-甘氨酸和3H-尿苷的掺入量会减少，降至未受压组织的50%以下。无论有无10%胎牛血清，375磅力/平方英寸的压力始终会使软骨合成活性提高10 - 15%。透析后的胎牛血清会使375磅力/平方英寸压力下的代谢反应从10%提高到55%。压力暴露后的恢复阶段包括一种释放（反弹）现象，即当组织受到抑制（75 - 300磅力/平方英寸）时，一阵代谢活动会将代谢率提高到正常水平，而在代谢已提高的组织（375磅力/平方英寸）中，会使代谢率加快60%。这些数据表明，关节软骨软骨细胞有能力将静水压力施加产生的机械信号迅速且有差异地转化为代谢事件。反应方向显然由施加力的大小和血清中可透析成分的存在决定。尽管施加的力只是部分模拟体内力，但观察到的对压力的反应支持了这样一种观点，即关节软骨代谢活动的压力调节可能是其维持的一个重要因素。
