Instituto Politécnico Nacional-Centro de Biotecnología Genómica, Boulevard del Maestro SN, Esq. Elías Piña, Col. Narciso Mendoza, 88710, Reynosa, Tamaulipas, Mexico.
Universidad Autónoma de Tamaulipas, Facultad de Medicina Veterinaria y Zootecnia, "Norberto Treviño Zapata", Carretera a Mante Km5, Victoria, Tamaulipas, Mexico.
Mol Biol Rep. 2020 Jan;47(1):87-95. doi: 10.1007/s11033-019-05110-0. Epub 2019 Oct 1.
Resequencing of Myostatin, Growth Hormone, Follistatin-A-like, Insulin-like Growth Factor I (IGF-I) and Myogenin (MYOG) genes was completed to discover novel genetic variations and assess non synonymous (ns) polymorphisms (SNPs) effect on growth related traits of channel catfish. Wild and farmed animals were used as a discovering population. Resequencing lead to the identification of 59 new variants in the five analyzed genes; 66% found in introns and 34% in coding regions. From coding regions, 14 variants were synonyms and six were ns variations. A mutation rate of one in 129 bp was estimated. Four ns variations were selected for validation and association analysis. In IGF-I two ns polymorphisms, at IGF-I19 the G wild type allele was fixed in population and for IGF-I63 the C allele had a frequency of 0.972 and for mutate allele G of 0.027. In MYOG two ns SNPs were assessed. MYOG131 presented a frequency of alleles T and A, of 0.754 and 0.246, respectively and MYOG233, with a frequency of G and C of 0.775 and 0.225, respectively. Only MYOG131 (g.529T>A) was significantly associated (P < 0.04) to some growth traits. Results suggest MYOG131 g.529T>A as candidate locus for genetic enhancement of growth traits in channel catfish.
对肌肉生长抑制素、生长激素、卵泡抑素 A 样蛋白、胰岛素样生长因子 I（IGF-I）和肌生成素（MYOG）基因进行重测序，以发现新的遗传变异，并评估非同义（ns）多态性（SNP）对斑点叉尾鮰生长相关性状的影响。野生和养殖动物被用作发现群体。重测序导致在这五个分析基因中鉴定出 59 个新变体；66%发现于内含子，34%发现于编码区。从编码区来看，14 个变体是同义的，6 个是 ns 变体。估计突变率为每 129bp 一个。选择了四个 ns 变体进行验证和关联分析。在 IGF-I 中，两个 ns 多态性，IGF-I19 的 G 野生型等位基因在群体中固定，而 IGF-I63 的 C 等位基因的频率为 0.972，而突变等位基因 G 的频率为 0.027。在 MYOG 中评估了两个 nsSNP。MYOG131 呈现等位基因 T 和 A 的频率分别为 0.754 和 0.246，而 MYOG233 则呈现等位基因 G 和 C 的频率分别为 0.775 和 0.225。只有 MYOG131（g.529T>A）与一些生长性状显著相关（P<0.04）。结果表明，MYOG131 g.529T>A 可能是斑点叉尾鮰生长性状遗传改良的候选基因座。
Zotero 插件