通过 CRISPR/Cas9 系统对受精卵进行基因编辑，生成肌肉生长抑制素基因编辑的斑点叉尾鮰（Ictalurus punctatus）。
Generation of Myostatin Gene-Edited Channel Catfish (Ictalurus punctatus) via Zygote Injection of CRISPR/Cas9 System.
机构信息
School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, Auburn, AL, 36849, USA.
Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
出版信息
Sci Rep. 2017 Aug 4;7(1):7301. doi: 10.1038/s41598-017-07223-7.
The myostatin (MSTN) gene is important because of its role in regulation of skeletal muscle growth in all vertebrates. In this study, CRISPR/Cas9 was utilized to successfully target the channel catfish, Ictalurus punctatus, muscle suppressor gene MSTN. CRISPR/Cas9 induced high rates (88-100%) of mutagenesis in the target protein-encoding sites of MSTN. MSTN-edited fry had more muscle cells (p < 0.001) than controls, and the mean body weight of gene-edited fry increased by 29.7%. The nucleic acid alignment of the mutated sequences against the wild-type sequence revealed multiple insertions and deletions. These results demonstrate that CRISPR/Cas9 is a highly efficient tool for editing the channel catfish genome, and opens ways for facilitating channel catfish genetic enhancement and functional genomics. This approach may produce growth-enhanced channel catfish and increase productivity.
肌肉生长抑制素（MSTN）基因在调节所有脊椎动物的骨骼肌生长中起着重要作用。在这项研究中，利用 CRISPR/Cas9 成功靶向了斑点叉尾鮰（Ictalurus punctatus）的肌肉抑制素基因 MSTN。CRISPR/Cas9 在 MSTN 的靶蛋白编码位点诱导了高突变率（88-100%）。与对照组相比，MSTN 编辑的鱼苗具有更多的肌肉细胞（p<0.001），并且基因编辑鱼苗的平均体重增加了 29.7%。与野生型序列相比，突变序列的核酸序列比对显示出多个插入和缺失。这些结果表明，CRISPR/Cas9 是编辑斑点叉尾鮰基因组的一种高效工具，并为促进斑点叉尾鮰遗传增强和功能基因组学开辟了途径。这种方法可能会产生生长增强的斑点叉尾鮰，提高生产力。
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