Cancer Research Unit, Department of Biology, University of York, York, UK.
Adv Exp Med Biol. 2019;1164:109-118. doi: 10.1007/978-3-030-22254-3_9.
Choosing an appropriate cell model(s) is the first decision to be made before starting a new project or programme of study. Here, we address the rationale that can be behind this decision and we summarize the current cell models that are used to study prostate cancer. Researchers face the challenge of choosing a model that recapitulates the complexity and heterogeneity of prostate cancer. The use of primary prostate epithelial cells cultured from patient tissue is discussed, and the necessity for close clinical-academic collaboration in order to do this is highlighted. Finally, a novel quantitative phase imaging technique is described, along with the potential for cell characterization to not only include gene expression and protein markers but also morphological features, cell behaviour and kinetic activity.
选择合适的细胞模型是开展新项目或研究计划前需要做出的首要决策。在此，我们将讨论做出这一决策背后的基本原理，并对目前用于研究前列腺癌的细胞模型进行总结。研究人员面临的挑战是选择能够重现前列腺癌复杂性和异质性的模型。本文讨论了从患者组织中培养原代前列腺上皮细胞的应用，并强调了为实现这一目标需要进行密切的临床-学术合作。最后，本文还介绍了一种新型定量相位成像技术，以及对细胞特征进行描述的潜力，不仅包括基因表达和蛋白标志物，还包括形态特征、细胞行为和动力学活性。
