Institute for Neurophysiology, Goethe University, Frankfurt, Germany.
Institute for Mathematics, Goethe University, Frankfurt, Germany.
Elife. 2019 Oct 3;8:e48408. doi: 10.7554/eLife.48408.
Functional diversity of midbrain dopamine (DA) neurons ranges across multiple scales, from differences in intrinsic properties and connectivity to selective task engagement in behaving animals. Distinct in vitro biophysical features of DA neurons have been associated with different axonal projection targets. However, it is unknown how this translates to different firing patterns of projection-defined DA subpopulations in the intact brain. We combined retrograde tracing with single-unit recording and labelling in mouse brain to create an in vivo functional topography of the midbrain DA system. We identified differences in burst firing among DA neurons projecting to dorsolateral striatum. Bursting also differentiated DA neurons in the medial substantia nigra (SN) projecting either to dorsal or ventral striatum. We found differences in mean firing rates and pause durations among ventral tegmental area (VTA) DA neurons projecting to lateral or medial shell of nucleus accumbens. Our data establishes a high-resolution functional in vivo landscape of midbrain DA neurons.
中脑多巴胺（DA）神经元的功能多样性跨越多个尺度，从内在特性和连接性的差异到行为动物的选择性任务参与。DA 神经元在体外具有不同的生物物理特征，与不同的轴突投射目标相关。然而，目前尚不清楚这如何转化为完整大脑中投射定义的 DA 亚群的不同放电模式。我们结合逆行追踪和小鼠大脑中的单细胞记录和标记，创建了中脑 DA 系统的体内功能图谱。我们发现投射到外侧纹状体的 DA 神经元的爆发放电存在差异。爆发也区分了投射到背侧或腹侧纹状体的内侧黑质（SN）中的 DA 神经元。我们发现投射到伏隔核外侧或内侧壳的腹侧被盖区（VTA）DA 神经元在平均放电率和停顿持续时间方面存在差异。我们的数据建立了中脑 DA 神经元的高分辨率功能体内图谱。
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