Department of Molecular Therapeutics, New York State Psychiatric Institute, New York, United States.
Department of Psychiatry, Columbia University, New York, United States.
Elife. 2018 Oct 8;7:e39786. doi: 10.7554/eLife.39786.
Dopamine neurons have different synaptic actions in the ventral and dorsal striatum (dStr), but whether this heterogeneity extends to dStr subregions has not been addressed. We have found that optogenetic activation of dStr dopamine neuron terminals in mouse brain slices pauses the firing of cholinergic interneurons in both the medial and lateral subregions, while in the lateral subregion the pause is shorter due to a subsequent excitation. This excitation is mediated mainly by metabotropic glutamate receptor 1 (mGluR1) and partially by dopamine D1-like receptors coupled to transient receptor potential channel 3 and 7. DA neurons do not signal to spiny projection neurons in the medial dStr, while they elicit ionotropic glutamate responses in the lateral dStr. The DA neurons mediating these excitatory signals are in the substantia nigra (SN). Thus, SN dopamine neurons engage different receptors in different postsynaptic neurons in different dStr subregions to convey strikingly different signals.
This article has been through an editorial process in which the authors decide how to respond to the issues raised during peer review. The Reviewing Editor's assessment is that all the issues have been addressed (see decision letter).
腹侧纹状体（dStr）中的多巴胺神经元具有不同的突触作用，但这种异质性是否扩展到 dStr 亚区尚未得到解决。我们发现，在小鼠脑切片中，光遗传学激活 dStr 多巴胺神经元末梢会使内侧和外侧亚区的胆碱能中间神经元的放电暂停，而在外侧亚区，由于随后的兴奋，暂停时间更短。这种兴奋主要由代谢型谷氨酸受体 1（mGluR1）介导，部分由与瞬时受体电位通道 3 和 7 偶联的多巴胺 D1 样受体介导。DA 神经元不会向内侧 dStr 的棘突投射神经元发出信号，而在外侧 dStr 会引起离子型谷氨酸反应。介导这些兴奋性信号的 DA 神经元位于黑质（SN）中。因此，SN 多巴胺神经元在不同的 dStr 亚区中与不同的突触后神经元结合不同的受体，传递截然不同的信号。
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