McIlwain D L, Farel P B
Brain Res. 1979 Dec 14;178(2-3):519-28. doi: 10.1016/0006-8993(79)90711-x.
The mitotic response of non-neuronal cells following motor axon transection was measured after in vitro incorporation of [3H]thymidine in frog spinal cord. This predominantly ipsilateral response occurs more rapidly and is of greater magnitude when motor axons are unilaterally transected at the ventral root than after sciatic nerve transection. No increase in incorporation occurred when regenerating fibers were transected a second time before reinnervation, but an increase was observed when the second operation was performed after the formation of functional neuromuscular connections had taken place. Autoradiographic studies after dorsal or ventral root transection showed that the distribution of labeled cells approximated the anatomical extent of the injured cellular elements within the spinal cord. These data are discussed in relation to the characteristics of the dividing cells and the nature of the events eliciting mitosis.
在蛙脊髓中体外掺入[3H]胸苷后，测量运动轴突横断后非神经元细胞的有丝分裂反应。当运动轴突在腹根处单侧横断时，这种主要为同侧的反应发生得更快，且幅度更大，比坐骨神经横断后的反应更明显。在再生纤维重新支配前再次横断时，掺入没有增加，但在功能性神经肌肉连接形成后进行第二次手术时则观察到增加。背根或腹根横断后的放射自显影研究表明，标记细胞的分布近似于脊髓内受损细胞成分的解剖范围。结合分裂细胞的特征和引发有丝分裂的事件的性质对这些数据进行了讨论。
