Arrieta Mikel, Colas Isabelle, Macaulay Malcolm, Waugh Robbie, Ramsay Luke
Cell and Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK.
Division of Plant Sciences, University of Dundee at The James Hutton Institute, Invergowrie, Dundee DD2 5DA, Scotland, UK.
Methods Mol Biol. 2020;2061:367-379. doi: 10.1007/978-1-4939-9818-0_26.
Determining when a barley plant starts and finishes meiosis is not trivial as when the spikelets undergo meiosis, the spike is not visible as it is still well within the leaf sheath on the developing tiller. This is a general constraint for any experiment involving meiosis, such as cytology, RNA extractions, or abiotic stress treatments aiming to target such a developmental stage. The lack of synchronicity between barley tillers within the same plant exacerbates the difficulty to determine the overall meiotic stage of a plant at a certain time.Given the lack of a nondestructive staging system for predicting the entry into meiosis and the problems of working with large pot plant systems, a modular plant growing is proposed. This system enables the growth of a high number of plants in a small surface, each producing a single tiller. The modular tray system was used to generate a nondestructive prediction tool for meiosis by using external morphological features. As an example, the system is used here for heat treating F plants in early meiosis stages to modify recombination.
确定大麦植株何时开始和结束减数分裂并非易事,因为当小穗进行减数分裂时,穗并不明显,因为它仍在发育中的分蘖的叶鞘内。这是任何涉及减数分裂的实验(如细胞学、RNA提取或旨在针对该发育阶段的非生物胁迫处理)的一个普遍限制。同一植株内大麦分蘖之间缺乏同步性加剧了在特定时间确定植株整体减数分裂阶段的难度。鉴于缺乏用于预测减数分裂开始的非破坏性分期系统以及处理大型盆栽植物系统的问题,提出了一种模块化植物种植方法。该系统能够在小面积内种植大量植株,每株产生一个分蘖。模块化托盘系统通过使用外部形态特征来生成减数分裂的非破坏性预测工具。例如,在此处使用该系统对处于减数分裂早期阶段的F植株进行热处理以改变重组。