Division of Tradition Korean Medicine Research, National Institute for Korean Medicine Development, Jangheung, Jeonnam, 59338, Republic of Korea; College of Veterinary Medicine BK21 Plus Project Team, Chonnam National University, Gwangju, 61186, Republic of Korea.
Division of Tradition Korean Medicine Research, National Institute for Korean Medicine Development, Jangheung, Jeonnam, 59338, Republic of Korea.
Regul Toxicol Pharmacol. 2019 Dec;109:104487. doi: 10.1016/j.yrtph.2019.104487. Epub 2019 Oct 1.
Cassia tora Linn. is an annual or perennial plant of the Fabaceae/Leguminosae family. It is used in traditional medicine for various biological activities including anti-constipation, anti-inflammatory, visual acuity, and hepato-protective activities. The present study was carried out to investigate the potential toxicity of C. tora L. seed ethanol extract (CTSEE) following a 13-week repeated oral administration to Sprague-Dawley rats. CTSEE was administered orally to male and female rats for 13 weeks at 0 (control), 500, 1000, and 2000 mg/kg/day (n = 10, for male and female rats for each dose). Additional recovery groups from the control group and high dose group were observed for a 4-week recovery period. At the end of the treatment and recovery periods, animals were sacrificed, and their organs were weighed and blood samples collected. There were no treatment-related adverse effects in clinical signs, body weight, food consumption, estrous cycle, sperm parameters, urinalysis, hematology, serum biochemistry, necropsy findings, organ weight, and histopathology at any doses tested. Under the present experimental conditions, the no-observed-adverse-effect level of the CTSEE was >2000 mg/kg/day in both genders, and no target organs were identified.
决明子是豆科植物，为一年生或多年生草本植物。在传统医学中，决明子被用于多种生物活性，包括抗便秘、抗炎、视力和保肝活性。本研究旨在研究决明子种子乙醇提取物（CTSEE）在连续口服给药 13 周后的潜在毒性。将 CTSEE 以 0（对照）、500、1000 和 2000mg/kg/天的剂量连续口服给予雄性和雌性大鼠，每周 6 天，每天 5 次，共 13 周（每组雄性和雌性大鼠各 10 只）。从对照组和高剂量组中还额外设置了恢复组，观察为期 4 周的恢复期。在治疗和恢复期结束时，处死动物，称重器官并采集血液样本。在任何测试剂量下，临床症状、体重、食物消耗、发情周期、精子参数、尿液分析、血液学、血清生化学、大体剖检结果、器官重量和组织病理学均无与处理相关的不良影响。在本实验条件下，CTSEE 在雌雄两性中的无明显不良作用水平（NOAEL）均>2000mg/kg/天，未鉴定出靶器官。
