Sala Laura, Chandrasekhar Srividya, Vidigal Joana A
Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, Bethesda, MD 20892, USA.
Laboratory of Biochemistry and Molecular Biology, National Cancer Institute, Bethesda, MD 20892, USA,
Front Biosci (Landmark Ed). 2020 Jan 1;25(1):1-42. doi: 10.2741/4793.
Argonaute (AGO) proteins play key roles in animal physiology by binding to small RNAs and regulating the expression of their targets. In mammals, they do so through two distinct pathways: the miRNA pathway represses genes through a multiprotein complex that promotes both decay and translational repression; the siRNA pathway represses transcripts through direct Ago2-mediated cleavage. Here, we review our current knowledge of mechanistic details and physiological requirements of both these pathways and briefly discuss their implications to human disease.
AGO蛋白通过与小RNA结合并调控其靶标的表达，在动物生理学中发挥关键作用。在哺乳动物中，它们通过两条不同的途径发挥作用：miRNA途径通过促进降解和翻译抑制的多蛋白复合体抑制基因；siRNA途径通过AGO2直接介导的切割抑制转录本。在这里，我们回顾了我们目前对这两条途径的机制细节和生理需求的了解，并简要讨论了它们对人类疾病的影响。
