Institute for the Application of Nuclear Energy (INEP), University of Belgrade, Belgrade, 381, Serbia.
Institute for the Application of Nuclear Energy (INEP), University of Belgrade, Belgrade, 381, Serbia.
Mech Ageing Dev. 2019 Oct;183:111151. doi: 10.1016/j.mad.2019.111151. Epub 2019 Oct 4.
Structural changes of glycans are observed in different (patho)physiological conditions. Human placental membrane (glyco)proteins were isolated from the first and third trimester placentas of mothers at different ages. By using lectin microarray, we demonstrated that the placental membrane N-glycome contains several N-glycan groups: high mannose, asialylated and sialylated biantennary moieties, bisected, core fucosylated, fucosylated at other positions (bearing terminal and/or antennary Fuc), α2-6 and α2-3 sialylated structures. Employing MALDI-TOF MS enabled identification of over sixty different N-glycan structures in all samples, with 17 moieties exceeding the relative abundance of 2%. The major MS peaks originated from: 1) biantennary complex type N-glycan with a bisecting GlcNAc residue and 2) a core Fuc paucimannosidic and high mannose type structures M3-M9. Age of mothers and the stage of placental development affected N-glycome. The work presented in this article is the first comprehensive mass spectrometric study of the N-glycome of human placental membrane proteins. Our results may be seen as the baseline which can serve for future MALDI MS profiling of the placental membrane N-glycome in different pathophysiological conditions.
在不同的（病理）生理条件下，聚糖的结构会发生变化。我们从不同年龄母亲的第一和第三个孕期胎盘分离出人类胎盘膜（糖）蛋白。通过使用凝集素微阵列，我们证明胎盘膜 N-聚糖包含几种 N-聚糖组：高甘露糖、去唾液酸和唾液酸化的双天线部分、双分岔、核心岩藻糖基化、在其他位置岩藻糖基化（带有末端和/或天线岩藻糖）、α2-6 和 α2-3 唾液酸化结构。使用 MALDI-TOF MS 能够鉴定所有样品中六十多种不同的 N-聚糖结构，其中 17 种结构的相对丰度超过 2%。主要 MS 峰源自：1）具有双分岔 GlcNAc 残基的双天线复合型 N-聚糖和 2）核心岩藻糖基化的低甘露糖和高甘露糖型结构 M3-M9。母亲的年龄和胎盘发育阶段影响了 N-聚糖。本文介绍的工作是对人类胎盘膜蛋白 N-聚糖进行的首次全面质谱研究。我们的结果可以被视为未来在不同病理生理条件下对胎盘膜 N-聚糖进行 MALDI MS 分析的基线。
