MUC2 基因甲基化检测在胰腺癌诊断中的意义。

Significance of MUC2 gene methylation detection in pancreatic cancer diagnosis.

机构信息

Department of Oncology, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou, 213003, China.

出版信息

Pancreatology. 2019 Dec;19(8):1049-1053. doi: 10.1016/j.pan.2019.09.012. Epub 2019 Sep 27.

DOI:10.1016/j.pan.2019.09.012

PMID:31590960

Abstract

PURPOSE

This study was conducted to explore the diagnostic value of MUC2 gene methylation in pancreatic cancer.

METHODS

Methylation restriction enzyme digestion (Msp I/Hap II) and polymerase chain reaction (PCR) were performed to detect methylation of the MUC2 gene in fecal and blood specimens from seven study subjects with pancreatic cancer (PC), chronic pancreatitis (CP), or normal controls (CON). Simultaneously, blood CA 19-9 levels were detected as a positive indicator of PC.

RESULTS

MUC2 methylation was detected in 50% of PC cell lines. In fecal samples, the MUC2 methylation rate in PC (n = 30) was 43.3%, which was significantly higher than those in CP (n = 8, 0%, P < 0.05) and CON (n = 20, 5.0%, P < 0.05). In blood samples, the MUC2 methylation rate in PC (n = 40) was 52.5%, which was significantly higher than those in CP (n = 15, 0%, P < 0.01) and CON (n = 25, 4.0%, P < 0.01). For PC diagnosis, MUC2 gene methylation in blood samples showed higher specificity and positive predictive value than CA 19-9. The combined detection in the feces and blood showed a 60% MUC2 methylation rate in PC (n = 10), which was higher than those in the CP (n = 5, 0%, P < 0.01) and CON (n = 12, 0%, P < 0.01).

CONCLUSIONS

The study can clearly indicate that combined detection of MUC2 gene methylation in the peripheral blood and feces could be used as a new screening and early diagnosis method for pancreatic cancer.

摘要

目的

本研究旨在探讨 MUC2 基因甲基化在胰腺癌诊断中的价值。

方法

采用甲基化限制性内切酶消化（Msp I/Hap II）和聚合酶链反应（PCR）检测 7 例胰腺癌（PC）、慢性胰腺炎（CP）和正常对照（CON）患者粪便和血液标本中 MUC2 基因的甲基化。同时，检测血液 CA 19-9 水平作为 PC 的阳性指标。

结果

MUC2 甲基化在 50%的 PC 细胞系中被检测到。在粪便样本中，PC（n=30）中 MUC2 甲基化率为 43.3%，明显高于 CP（n=8，0%，P<0.05）和 CON（n=20，5.0%，P<0.05）。在血液样本中，PC（n=40）中 MUC2 甲基化率为 52.5%，明显高于 CP（n=15，0%，P<0.01）和 CON（n=25，4.0%，P<0.01）。对于 PC 诊断，血液样本中 MUC2 基因甲基化的特异性和阳性预测值均高于 CA 19-9。粪便和血液联合检测显示 PC（n=10）中 MUC2 甲基化率为 60%，明显高于 CP（n=5，0%，P<0.01）和 CON（n=12，0%，P<0.01）。