Al-Shibli Saad M, Harun Norra, Ashour Abdelkader E, Mohd Kasmuri Mohd Hanif B, Mizan Shaikh
Department of Basic Medical Sciences, International Islamic University, Kuantan, Pahang, Malaysia.
Pathology Department, Hospital Tengku Ampuan Afzan, Kuantan, Pahang, Malaysia.
PeerJ. 2019 Oct 2;7:e7624. doi: 10.7717/peerj.7624. eCollection 2019.
Obesity is demonstrated to be a risk factor in the development of cancers of various organs, such as colon, prostate, pancreas and so on. Leptine (LEP) is the most renowned of the adipokines. As a hormone, it mediates its effect through leptin receptor (LEPR), which is widely expressed in various tissues including colon mucosa. In this study, we have investigated the degree of expression of LEP and LEPR in colorectal cancer (CRC). We collected 44 surgically resected colon cancer tissues along with normal adjacent colon tissue (NACT) from a sample of CRC patients from the Malaysian population and looked for leptin and leptin receptors using immunohistochemistry (IHC). All the samples showed low presence of both LEP and LEPR in NACT, while both LEP and LEPR were present at high intensity in the cancerous tissues with 100% and 97.7% prevalence, respectively. Both were sparsed in the cytoplasm and were concentrated beneath the cell membrane. However, we did not find any significant correlation between their expression and pathological parameters like grade, tumor size, and lymph node involvement. Our study further emphasizes the possible causal role of LEP and LEPR with CRC, and also the prospect of using LEPR as a possible therapeutic target.
肥胖被证明是多种器官癌症发生发展的一个风险因素，如结肠癌、前列腺癌、胰腺癌等。瘦素（LEP）是最著名的脂肪因子。作为一种激素，它通过瘦素受体（LEPR）介导其作用，LEPR在包括结肠黏膜在内的各种组织中广泛表达。在本研究中，我们调查了结直肠癌（CRC）中LEP和LEPR的表达程度。我们从马来西亚人群的CRC患者样本中收集了44例手术切除的结肠癌组织以及正常相邻结肠组织（NACT），并使用免疫组织化学（IHC）检测瘦素和瘦素受体。所有样本在NACT中LEP和LEPR的表达均较低，而在癌组织中LEP和LEPR均呈高强度表达，患病率分别为100%和97.7%。两者均在细胞质中稀疏分布，并集中在细胞膜下方。然而，我们没有发现它们的表达与分级、肿瘤大小和淋巴结受累等病理参数之间存在任何显著相关性。我们的研究进一步强调了LEP和LEPR与CRC可能的因果关系，以及将LEPR作为一种可能的治疗靶点的前景。
