Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
J Feline Med Surg. 2020 Aug;22(8):805-808. doi: 10.1177/1098612X19880632. Epub 2019 Oct 8.
The aim of this study was to evaluate the diagnostic concordance between the toothbrush and carpet techniques for the detection of in cats in a field study.
Thirty-nine Persian cats from a cattery were used. Fungal culture samples from the haircoat of each cat were collected by stroking the coat with a sterile toothbrush and a 5 × 5 cm-sized sterile carpet square (n = 78 total samples). Specimens were inoculated onto Mycosel Agar and incubated at 25°C for 21 days. Both techniques were compared using the following parameters: number of plates without fungal growth, number of plates with contaminant growth and number of plates positive for dermatophytes.
The feline population in the study cattery was 39. Thirty (77%) were symptomatic and nine (23%) asymptomatic. The diagnosis was made via carpet and toothbrush methods and 78 cultures were performed. On day 21, was detected in all culture plates. No contaminant molds were observed.
The concordance rate between the carpet and toothbrush techniques among the 78 evaluable culture plates was 100%. Both methods are equally effective for collecting material for culture. Additionally, both techniques are inexpensive and easy to perform in feline clinical practice.
本研究旨在评估牙刷和地毯技术在猫中检测 的诊断一致性。
从一家繁殖场使用了 39 只波斯猫。通过用无菌牙刷和 5×5 厘米大小的无菌地毯方块刷拭毛发,收集每只猫的毛发样本进行真菌培养(总共收集了 78 个样本)。将标本接种到 Mycosel 琼脂上,在 25°C 下孵育 21 天。使用以下参数比较两种技术:无真菌生长的平板数量、有污染物生长的平板数量和有皮肤真菌生长的平板数量。
研究繁殖场的猫群有 39 只。其中 30 只(77%)有症状,9 只(23%)无症状。通过地毯和牙刷方法进行诊断,并进行了 78 次培养。第 21 天,所有培养平板均检测到 。未观察到污染霉菌。
在可评估的 78 个培养平板中,地毯和牙刷技术之间的一致性率为 100%。两种方法对于收集真菌培养物都是同样有效的。此外,两种技术在猫科临床实践中都既便宜又易于实施。