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天然淀粉和改性淀粉对大豆蛋白凝胶的质构、流变学和微观结构特性的影响。

Effects of native starch and modified starches on the textural, rheological and microstructural characteristics of soybean protein gel.

机构信息

State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250353, China; College of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250353, China.

College of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong 250353, China.

出版信息

Int J Biol Macromol. 2020 Jan 1;142:237-243. doi: 10.1016/j.ijbiomac.2019.09.095. Epub 2019 Oct 5.

Abstract

The effects of native starch (NS), acetylated starch (AS), and acetylated distarch phosphate (ADSP) on the gel properties of soybean protein thermal gel were investigated using texture analysis, low-field nuclear magnetic resonance (LF-NMR) spectroscopy, dynamic rheometry and scanning electron microscopy. The results of the textural profile analysis showed that 10% ADSP increased the hardness and chewiness of the mixed gel, while NS and AS led to decreases in the textural properties. The results of the LF-NMR analysis indicated that the AS improved the water-holding capacity of the mixed gel due to the transformation of weakly bound water to strongly bound water. During heating and cooling, the rheological profiles of the elastic (G') and viscous modulus (G″) of all the samples exhibited a two-stage pattern of decrease and then increase, and the final values of G' and G″ reached maxima when the ADSP content was 10%. The scanning electron microscopy images showed that the ADSP granules dispersed in the gel network. The integrity of the starch granules was crucial for regulating the properties of the soybean protein gel. These results provided information about the further design and preparation of soybean protein foods containing modified starch.

摘要

采用质构分析、低场核磁共振(LF-NMR)光谱、动态流变学和扫描电子显微镜研究了原淀粉(NS)、乙酰化淀粉(AS)和乙酰化双淀粉磷酸酯(ADSP)对大豆蛋白热凝胶凝胶性能的影响。质构分析结果表明,10%的 ADSP 增加了混合凝胶的硬度和咀嚼性,而 NS 和 AS 则降低了凝胶的质构特性。LF-NMR 分析结果表明,AS 提高了混合凝胶的持水能力,这是由于弱结合水向强结合水的转化。在加热和冷却过程中,所有样品的弹性(G')和粘性模量(G″)流变曲线呈现先下降后上升的两阶段模式,当 ADSP 含量为 10%时,G'和 G″的最终值达到最大值。扫描电子显微镜图像显示,ADSP 颗粒分散在凝胶网络中。淀粉颗粒的完整性对于调节大豆蛋白凝胶的性能至关重要。这些结果为进一步设计和制备含有改性淀粉的大豆蛋白食品提供了信息。

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