Oeda K, Sakaki T, Ohkawa H
DNA. 1985 Jun;4(3):203-10. doi: 10.1089/dna.1985.4.203.
Rat liver cytochrome P-450MC cDNA was inserted between the ADH1 promoter and terminator regions of the yeast expression vector pAAH5. On introduction of the resulting recombinant plasmid pAMC1, Saccharomyces cerevisiae cells synthesized up to 8 X 10(5) molecules per cell of the cytochrome P-450MC protein, most of which was localized in yeast microsomes. Approximately half of the synthesized cytochrome contained heme in the enzyme molecule. These formed a functional electron-transport chain in the microsomes which exhibited aryl hydrocarbon hydroxylase activity toward benzo[a]pyrene.
将大鼠肝脏细胞色素P - 450MC cDNA插入酵母表达载体pAAH5的ADH1启动子和终止子区域之间。导入所得重组质粒pAMC1后，酿酒酵母细胞每个细胞可合成多达8×10⁵个细胞色素P - 450MC蛋白分子，其中大部分定位于酵母微粒体中。所合成的细胞色素中约一半在酶分子中含有血红素。这些在微粒体中形成了一个功能性电子传递链，该电子传递链对苯并[a]芘表现出芳烃羟化酶活性。
