Hathcock K S, Kenny J J, Hodes R J
Eur J Immunol. 1985 Jun;15(6):564-9. doi: 10.1002/eji.1830150607.
The requirement for idiotype-specific T cells was investigated in the T15 idiotype-dominant T cell-dependent response of unprimed BALB/c and (BALB/c X C57BL/6)F1 B cells to phosphorylcholine (PC). It was first demonstrated that cloned keyhole limpet hemocyanin (KLH)-specific, major histocompatibility complex (MHC)-restricted T helper (Th) cells as well as heterogeneous KLH-primed Th populations were capable of generating PC-specific antibody responses in T-depleted unprimed B cell populations cultured in the presence of PC-KLH. The PC-binding antibody responses generated under these conditions were indistinguishable when assayed for carrier-hapten linkage requirements, immunoglobulin isotype (predominantly IgM) or PC affinity. Further, it was observed that the PC-binding antibodies which were generated in the presence of these two T cell populations expressed equivalently high levels of T15 idiotype. Assaying antibody and idiotype by either enzyme-linked immunosorbent assay or plaque-forming cell assay yielded similar results. Since monoclonal MHC-restricted, KLH-specific Th cells presumably lack any additional T cell populations, these results argue against an absolute requirement for anti-idiotypic Th cells in the generation of T15-dominant antibody responses.
在未致敏的BALB/c和（BALB/c×C57BL/6）F1 B细胞对磷酸胆碱（PC）的T15独特型主导的T细胞依赖性反应中，研究了对独特型特异性T细胞的需求。首先证明，克隆的钥孔戚血蓝蛋白（KLH）特异性、主要组织相容性复合体（MHC）限制性T辅助（Th）细胞以及异质性的经KLH致敏的Th细胞群体，能够在PC-KLH存在下培养的无T细胞的未致敏B细胞群体中产生PC特异性抗体反应。当检测载体-半抗原连接需求、免疫球蛋白同种型（主要是IgM）或PC亲和力时，在这些条件下产生的PC结合抗体反应没有区别。此外，观察到在这两种T细胞群体存在的情况下产生的PC结合抗体表达了同等高水平的T15独特型。通过酶联免疫吸附测定或空斑形成细胞测定来检测抗体和独特型，得到了相似的结果。由于单克隆MHC限制性、KLH特异性Th细胞大概缺乏任何其他T细胞群体，这些结果反对在产生T15主导的抗体反应中对抗独特型Th细胞的绝对需求。
