Erb P, Ramila G, Sklenar I, Kennedy M, Sunshine G H
Immunobiology. 1985 May;169(4):424-35. doi: 10.1016/S0171-2985(85)80022-X.
Dendritic cells and macrophages obtained from spleen and peritoneal exudate were tested as accessory cells for the activation of lymphokine production by T cells, for supporting T-B cooperation and for the induction of antigen-specific T helper cells. Dendritic cells as well as macrophages were able to activate T cells for interleukin-2 secretion and functioned as accessory cells in T-B cooperation, but only macrophages induced T helper cells, which cooperate with B cells by a linked recognition interaction, to soluble antigens. Dendritic cell- and antigen-activated T cells also did not help B cells in the presence of Con A supernatants which contained various T cell- and B cell-stimulatory factors. The failure of dendritic cells to differentiate memory into functional T helper cells, but their efficient accessory cell function in T-B cooperation, where functional T helper cells are already present, can be best explained by a differential accessory cell requirement for T helper cell activation dependent on the differentiation stage of the T helper cell.
从脾脏和腹腔渗出液中获得的树突状细胞和巨噬细胞作为辅助细胞进行了测试，用于激活T细胞产生淋巴因子、支持T-B协作以及诱导抗原特异性T辅助细胞。树突状细胞和巨噬细胞都能够激活T细胞分泌白细胞介素-2，并在T-B协作中作为辅助细胞发挥作用，但只有巨噬细胞能诱导T辅助细胞，这些T辅助细胞通过连锁识别相互作用与B细胞协同作用于可溶性抗原。在含有各种T细胞和B细胞刺激因子的刀豆蛋白A上清液存在的情况下，树突状细胞和抗原激活的T细胞也无助于B细胞。树突状细胞未能分化为功能性T辅助记忆细胞，但其在已经存在功能性T辅助细胞的T-B协作中具有有效的辅助细胞功能，这可以通过依赖于T辅助细胞分化阶段的T辅助细胞激活对辅助细胞的不同需求来最好地解释。
