Nakai H, Taylor A L
J Bacteriol. 1985 Jul;163(1):282-90. doi: 10.1128/jb.163.1.282-290.1985.
Bacteriophage Mu DNA integration in Escherichia coli strains infected after alignment of chromosomal replication was analyzed by a sandwich hybridization assay. The results indicated that Mu integrated into chromosomal segments at various distances from oriC with similar kinetics. In an extension of these studies, various Hfr strains were infected after alignment of chromosomal replication, and Mu transposition was shut down early after infection. The positions of integrated Mu copies were inferred from the transfer kinetics of Mu to an F- strain. Our analysis indicated that the location of Mu DNA in the host chromosome was not dependent on the positions of host replication forks at the time of infection. However, the procedure for aligning chromosomal replication affected DNA transfer by various Hfr strains differently, and this effect could account for prior results suggesting preferential integration of Mu at host replication forks.
通过夹心杂交分析，研究了在染色体复制对齐后感染的大肠杆菌菌株中噬菌体Mu DNA的整合情况。结果表明，Mu以相似的动力学整合到距离oriC不同距离的染色体片段中。在这些研究的扩展中，在染色体复制对齐后感染了各种Hfr菌株，并在感染后早期关闭了Mu转座。从Mu转移到F-菌株的动力学推断出整合的Mu拷贝的位置。我们的分析表明，宿主染色体中Mu DNA的位置不依赖于感染时宿主复制叉的位置。然而，对齐染色体复制的程序对各种Hfr菌株的DNA转移影响不同，这种影响可以解释先前表明Mu在宿主复制叉处优先整合的结果。
