Otake Mao, Ukita Yoshiaki
Faculty of Engineering, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, University of Yamanashi.
Anal Sci. 2019;35(10):1123-1127. doi: 10.2116/analsci.19P137.
Measurement of single molecule interaction is performed by various micromanipulation techniques. However, a complex operation is required to perform the measurement, so the measurement throughput is low and a large amount of time is required for statistical analysis. In this paper, we propose a method for measuring intermolecular interactions that applies a tensile force to the intermolecular bond by using centrifugal force and can easily measure binding force. The binding force of an antigen-antibody reaction system was measured by the use of the proposed method as a proof of concept. The measured binding forces were distributed in the range of 19 - 133 pN. The values obtained by use of the proposed method were consistent with the conventional method. This result is promising for applications that require further development, such as the development of a biosensor as a novel measuring technique that is based on the intermolecular interaction measurement.
单分子相互作用的测量是通过各种微操纵技术来进行的。然而，进行测量需要复杂的操作，因此测量通量较低，并且统计分析需要大量时间。在本文中，我们提出了一种测量分子间相互作用的方法，该方法通过利用离心力对分子间键施加拉力，并且能够轻松测量结合力。作为概念验证，使用所提出的方法测量了抗原 - 抗体反应系统的结合力。测得的结合力分布在19 - 133 pN的范围内。使用所提出的方法获得的值与传统方法一致。这一结果对于需要进一步发展的应用是有前景的，例如作为基于分子间相互作用测量的新型测量技术的生物传感器的开发。
