Senior P V, Pritchett C J, Sunter J P, Appleton D R, Watson A J
J Pathol. 1985 May;146(1):39-49. doi: 10.1002/path.1711460105.
When a segment of small intestine is transplanted to the external abdominal wall in rats adaptive changes occur in the exposed mucosa. These probably represent an extreme example of a physiological response to one type of trophic influence--the effect of mechanical trauma. The nature of the changes has been studied at 7 weeks after externalization using simple morphometry and a number of cytokinetic techniques (thymidine labelling, vincristine-induced metaphase arrest and the fraction-of-labelled-mitoses method), and comparisons drawn with the normal ileum. The exteriorized mucosa showed marked villus atrophy and hyperplasia of the crypts to three times normal size as a result of increases both in crypt length and crypt circumference. Neither metaplastic nor dysplastic epithelial abnormalities were observed. Crypt-cell production rate doubled in the hyperplastic crypts due to an increase in the size of the proliferation zone within the crypt, and the distribution of proliferating cells within the crypt changed. But cell cycle times were prolonged and more maturing cells were retained in the hyperplastic crypts. The potential usefulness of this model, particularly in carcinogenicity studies is considered.
当将一段小肠移植到大鼠腹壁外时，暴露的黏膜会发生适应性变化。这些变化可能代表了对一种营养影响——机械创伤效应的生理反应的极端例子。在小肠外置7周后，使用简单的形态测量法和一些细胞动力学技术（胸腺嘧啶核苷标记、长春新碱诱导的中期阻断以及标记有丝分裂分数法）对这些变化的性质进行了研究，并与正常回肠进行了比较。外置的黏膜显示出明显的绒毛萎缩，隐窝增生至正常大小的三倍，这是由于隐窝长度和隐窝周长均增加所致。未观察到化生或发育异常的上皮异常情况。由于隐窝内增殖区大小增加，增生隐窝中的隐窝细胞产生率加倍，并且隐窝内增殖细胞的分布发生了变化。但是细胞周期时间延长，更多成熟细胞保留在增生的隐窝中。考虑了该模型的潜在用途，特别是在致癌性研究中的用途。
