Kikuchi A, Flamm E, Weisberg R A
J Mol Biol. 1985 May 25;183(2):129-40. doi: 10.1016/0022-2836(85)90207-4.
We report the isolation of mutations in, and the characterization of, an Escherichia coli gene, hip, that is required for site-specific recombination of phage lambda. hip mutants are recessive and are located near minute 20 on the linkage map. The gene product is not vital to bacterial growth, since deletion mutants are viable. The absence of hip product reduces lambda integration to barely detectable levels and also reduces prophage excision, but less drastically. Certain mutations in the lambda int gene partially restore integration and excision in hip- hosts. Homologous recombination promoted by recA does not require hip function. In addition to their defect in site-specific recombination, hip mutants are unable to support lytic growth of phage Mu or of certain lambda mutants. Their pleiotropic phenotype closely resembles that of himA mutants, but complementation, mapping and DNA sequencing show that hip and himA are different genes.
我们报告了对大肠杆菌中一个基因hip的突变分离及特性研究，该基因是噬菌体λ特异性位点重组所必需的。hip突变体是隐性的，位于连锁图上20分钟附近。该基因产物对细菌生长并非至关重要，因为缺失突变体是可行的。hip产物的缺失将λ整合降低到几乎检测不到的水平，也会降低原噬菌体切除，但程度较轻。λ int基因中的某些突变可部分恢复hip-宿主中的整合和切除。recA促进的同源重组不需要hip功能。除了在特异性位点重组方面存在缺陷外，hip突变体无法支持噬菌体Mu或某些λ突变体的裂解生长。它们的多效表型与himA突变体非常相似，但互补、定位和DNA测序表明hip和himA是不同的基因。
