Department of Breast, Thyroid Surgery, Research Institute of Surgery, Daping Hospital, Army Military Medical University, Chongqing, China.
Eur Rev Med Pharmacol Sci. 2019 Sep;23(18):7989-7999. doi: 10.26355/eurrev_201909_19015.
The important role of microRNA-1271 (miR-1271) has been identified in human diseases and cancers. However, the biological function of miR-1271 remains ambiguous in papillary thyroid carcinoma (PTC). Therefore, the specific role of miR-1271 was investigated in PTC.
The expressions of miR-1271 and insulin receptor substrate 1 (IRS1) were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) assay. The protein expression of the genes was measured by Western blot analysis. The function of miR-1271 was investigated using methyl thiazolyl tetrazolium (MTT) and transwell assays. The Dual-Luciferase assay was used to observe the relationship between miR-1271 and IRS1.
MiR-1271 was downregulated in PTC tissues. Moreover, overexpression of miR-1271 suppressed migration, invasion and proliferation of PTC cells. Furthermore, IRS1 was indicated as a direct target gene of miR-1271 and knockdown of IRS1 inhibited cell migration, invasion and proliferation in PTC. In addition, miR-1271 inhibited the progression of PTC by targeting IRS1. Besides that, miR-1271 blocked the epithelial-mesenchymal transition (EMT) and protein kinase B (AKT) pathway in PTC.
MiR-1271 inhibited the progression of PTC by targeting IRS1 and blocking EMT and AKT pathway.
微小 RNA-1271（miR-1271）在人类疾病和癌症中的重要作用已得到证实。然而，miR-1271 在甲状腺乳头状癌（PTC）中的生物学功能仍不明确。因此，本研究旨在探讨 miR-1271 在 PTC 中的具体作用。
通过实时定量聚合酶链反应（qRT-PCR）检测 miR-1271 和胰岛素受体底物 1（IRS1）的表达。通过 Western blot 分析测量基因的蛋白表达。使用甲基噻唑基四唑（MTT）和 Transwell 测定法研究 miR-1271 的功能。双荧光素酶报告基因实验观察 miR-1271 与 IRS1 之间的关系。
miR-1271 在 PTC 组织中表达下调。此外，过表达 miR-1271 抑制 PTC 细胞的迁移、侵袭和增殖。进一步研究表明，IRS1 是 miR-1271 的直接靶基因，敲低 IRS1 抑制 PTC 细胞的迁移、侵袭和增殖。此外，miR-1271 通过靶向 IRS1 抑制 PTC 的进展。除此之外，miR-1271 阻断了 PTC 中的上皮间质转化（EMT）和蛋白激酶 B（AKT）通路。
miR-1271 通过靶向 IRS1 并阻断 EMT 和 AKT 通路抑制 PTC 的进展。
