微小 RNA-1271 通过靶向 IRS1 并使 AKT 通路失活抑制甲状腺乳头状癌的进展。
MicroRNA-1271 inhibits the progression of papillary thyroid carcinoma by targeting IRS1 and inactivating AKT pathway.
机构信息
Department of Breast, Thyroid Surgery, Research Institute of Surgery, Daping Hospital, Army Military Medical University, Chongqing, China.
出版信息
Eur Rev Med Pharmacol Sci. 2019 Sep;23(18):7989-7999. doi: 10.26355/eurrev_201909_19015.
OBJECTIVE
The important role of microRNA-1271 (miR-1271) has been identified in human diseases and cancers. However, the biological function of miR-1271 remains ambiguous in papillary thyroid carcinoma (PTC). Therefore, the specific role of miR-1271 was investigated in PTC.
PATIENTS AND METHODS
The expressions of miR-1271 and insulin receptor substrate 1 (IRS1) were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) assay. The protein expression of the genes was measured by Western blot analysis. The function of miR-1271 was investigated using methyl thiazolyl tetrazolium (MTT) and transwell assays. The Dual-Luciferase assay was used to observe the relationship between miR-1271 and IRS1.
RESULTS
MiR-1271 was downregulated in PTC tissues. Moreover, overexpression of miR-1271 suppressed migration, invasion and proliferation of PTC cells. Furthermore, IRS1 was indicated as a direct target gene of miR-1271 and knockdown of IRS1 inhibited cell migration, invasion and proliferation in PTC. In addition, miR-1271 inhibited the progression of PTC by targeting IRS1. Besides that, miR-1271 blocked the epithelial-mesenchymal transition (EMT) and protein kinase B (AKT) pathway in PTC.
CONCLUSIONS
MiR-1271 inhibited the progression of PTC by targeting IRS1 and blocking EMT and AKT pathway.
目的
微小 RNA-1271（miR-1271）在人类疾病和癌症中的重要作用已得到证实。然而，miR-1271 在甲状腺乳头状癌（PTC）中的生物学功能仍不明确。因此，本研究旨在探讨 miR-1271 在 PTC 中的具体作用。
患者与方法
通过实时定量聚合酶链反应（qRT-PCR）检测 miR-1271 和胰岛素受体底物 1（IRS1）的表达。通过 Western blot 分析测量基因的蛋白表达。使用甲基噻唑基四唑（MTT）和 Transwell 测定法研究 miR-1271 的功能。双荧光素酶报告基因实验观察 miR-1271 与 IRS1 之间的关系。
结果
miR-1271 在 PTC 组织中表达下调。此外，过表达 miR-1271 抑制 PTC 细胞的迁移、侵袭和增殖。进一步研究表明，IRS1 是 miR-1271 的直接靶基因，敲低 IRS1 抑制 PTC 细胞的迁移、侵袭和增殖。此外，miR-1271 通过靶向 IRS1 抑制 PTC 的进展。除此之外，miR-1271 阻断了 PTC 中的上皮间质转化（EMT）和蛋白激酶 B（AKT）通路。
结论
miR-1271 通过靶向 IRS1 并阻断 EMT 和 AKT 通路抑制 PTC 的进展。
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