Licon Katherine, Shen John Paul, Munson Brenton P, Michaca Manuel, Fassino Cole, Fassino Luke, Kreisberg Jason F, Ideker Trey
Department of Medicine, UC San Diego, La Jolla, CA, USA.
Department of Bioengineering, UC San Diego, La Jolla, CA, USA.
Methods Mol Biol. 2019;2049:73-85. doi: 10.1007/978-1-4939-9736-7_4.
Systematic measurements of genetic interactions have been used to classify gene functions and to categorize genes into protein complexes, functional pathways and biological processes. This protocol describes how to perform a high-throughput genetic interaction screen in S. cerevisiae using a variant of epistatic miniarray profiles (E-MAP) in which the fitnesses of 6144 colonies are measured simultaneously. We also describe the computational methods to analyze the resulting data.
系统的遗传相互作用测量已被用于对基因功能进行分类，并将基因归类为蛋白质复合物、功能途径和生物过程。本方案描述了如何在酿酒酵母中使用上位性微阵列图谱（E-MAP）的一种变体进行高通量遗传相互作用筛选，其中可同时测量6144个菌落的适应性。我们还描述了分析所得数据的计算方法。
