DSM Nutritional Products Ltd., Kaiseraugst, Switzerland.
AVR Consulting Ltd., Northwich, UK.
Int J Cosmet Sci. 2019 Dec;41(6):624-628. doi: 10.1111/ics.12585.
Plasmin, a relatively unspecific trypsin-like serine protease, is involved in many physiological and pathological conditions, particularly in dermatoses with barrier impairment. It is secreted as the inactive zymogen plasminogen and is activated to plasmin by plasminogen activators, such as urokinase. There still exists a paucity of data on the precise localization of epidermal plasmin(ogen) within the epidermis and the stratum corneum. The aim of the present study was to get information about its origin and ultrastructural localization within normal human epidermis.
We performed immunoelectron transmission electron microscopy immunogold labelling in normal abdominal human skin.
Plasmin was only observed in the terminally differentiated cell layers of the epidermis and was largely associated with the corneocyte envelopes and to some extent with the intercellular lipid matrix in the stratum corneum.
Our results indicate that in normal human skin, plasmin(ogen) is synthesized by differentiated epidermal keratinocytes of the stratum granulosum and is not serum-born.
纤溶酶是一种相对非特异性的胰蛋白酶样丝氨酸蛋白酶，参与多种生理和病理状态，特别是在屏障功能受损的皮肤病中。它以无活性的酶原纤溶酶原的形式分泌，并被纤溶酶原激活物（如尿激酶）激活为纤溶酶。关于表皮纤溶酶（原）在表皮和角质层中的精确定位，目前仍缺乏数据。本研究旨在获取有关其在正常人体表皮中的起源和超微结构定位的信息。
我们在正常腹部人体皮肤中进行了免疫电镜透射电子显微镜免疫金标记。
纤溶酶仅在表皮的终末分化细胞层中观察到，主要与角质细胞包膜相关，在一定程度上与角质层中的细胞间脂质基质相关。
我们的结果表明，在正常人体皮肤中，纤溶酶原由颗粒层的分化表皮角朊细胞合成，并非来自血清。
