Su Dongdong, Cheng Dan, Lv Yun, Ren Xiaojun, Wu Qian, Yuan Lin
Department of Chemistry and Chemical Engineering, Beijing University of Technology, Beijing, 100124, PR China.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Feb 5;226:117635. doi: 10.1016/j.saa.2019.117635. Epub 2019 Oct 8.
Hydrogen sulfide (HS) has been found to be an important biological regulator that plays important roles in many physiological and pathological processes. Near-infrared (NIR) fluorescent probes capable of selectively detecting HS in vivo will be useful tools to understand its mechanisms in biological processes. Herein, we reported an easily synthesized and stimuli-responsive NIR fluorescent probe (QCy7-HS) for selective evaluation of endogenous HS in the living cells and mice. In response to cellular HS stimulus, QCy7-HS is converted to QCy7 and shows a unique off-on near-infrared fluorescence signal change. The results of selectivity and kinetic studies indicated that our probe has high HS binding capacity. Therefore, this probe was used for the fluorescence detection of HS in cells. Moreover, the probe was applied for study exogenous and endogenous HS in live mice, indicating that the new probe can be used as an efficient tool on HS related events in living animals.
硫化氢（HS）已被发现是一种重要的生物调节剂，在许多生理和病理过程中发挥着重要作用。能够在体内选择性检测HS的近红外（NIR）荧光探针将成为了解其在生物过程中作用机制的有用工具。在此，我们报道了一种易于合成且对刺激有响应的近红外荧光探针（QCy7-HS），用于选择性评估活细胞和小鼠体内的内源性HS。响应细胞HS刺激时，QCy7-HS会转化为QCy7，并显示出独特的关闭-开启近红外荧光信号变化。选择性和动力学研究结果表明，我们的探针具有高HS结合能力。因此，该探针用于细胞中HS的荧光检测。此外，该探针还应用于活体小鼠中外源性和内源性HS的研究，表明这种新型探针可作为研究活体动物中与HS相关事件的有效工具。
