Wu Yuan-Jun, Wu Yong, Li Hui-Sen, Chen Bao-Chan, Liu Yan, Wu Yue-Qin, Liang Si-Yuan, Liang Mei-Qi
Department of Blood Transfusion, Dongguan Maternal and Child Health Care Hospital. Dongguan 523120, Guangdong Province, China,E-mail:
Department of Blood Transfusion, Dongguan Donghua Hospital, Dongguan 523110, Guangdong Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2019 Oct;27(5):1607-1611. doi: 10.19746/j.cnki.issn.1009-2137.2019.05.038.
To explore the effect of high volume platelet reduction therapy on the white blood cell (WBC) count and hemoglobin (Hb) level in patients with thrombocytosis.
Thirty-two plateletphoreses were performed for patients with thromocytosis by using ELP or MNC program of blood component isolator of COBE spectra continuous flow concentrifugation and the ACD-A preservation solution for blood as blood anticoagulant. In each treatment of patients, 2.5-3.0 tines total blood volume (TBV) were circulated, then the platelet suspension of 1/5-1/4 time TBV was prepared and collected.
A single plateletpheresis took (212.53±41.54) minutes in which (8 812.63±2087.15) ml blood were treated, and (798.84±190.77) ml platelet suspension was collected. In the suspension, the platelet count was 4 486.50 (3 058.50-5 279.50)×10/L, containing 3 455.50 (2 288.68-4 226.71)×10. WBC count was 13.79 (10.21-20.72)×10/L, containing 11.90(7.81-14.40)×10. Hemoglobin concentration was (3.28±1.25) g/L，containing (2.62 ± 1.17) g. Before and after plateletpheresis, the patients' platelet count was 1 263.00 (1 052.50-1 807.50)×10/L and (778.83±247.25)×10/L（Z=4.94, P＜0.01), WBC count was 9.96(6.44-14.01)×10/L and 8.59(5.37, 13.12)×10/L (Z=13.31, P＜0.05), Hemoglobin concentration was (112.63 ± 24.56)g/L and (109.55 ± 24.46)g/L (t=1.68，P＞0.05).
Using continuous flow centrifugation and blood component separating in plateletpheresis for the patients with thrombocytosis can obviously decrease the high ratio of platelets, and improve the effect of plateletpheresis. The high volume platelet reduction therapy can lead to decrease of WBC count to some alent, degree but WBC count still in the normal range, moreover not affect the hemoglobin level significantly.
探讨高容量血小板单采术对血小板增多症患者白细胞（WBC）计数及血红蛋白（Hb）水平的影响。
采用COBE spectra连续流动离心式血液成分分离机的ELP或MNC程序及ACD - A血液保存液作为血液抗凝剂，对血小板增多症患者进行32次血小板单采术。每次治疗使患者2.5 - 3.0倍全血容量（TBV）循环，然后制备并采集1/5 - 1/4倍TBV的血小板悬液。
单次血小板单采耗时（212.53±41.54）分钟，处理血液量为（8812.63±2087.15）ml，采集血小板悬液量为（798.84±190.77）ml。悬液中血小板计数为4486.50（3058.50 - 5279.50）×10⁹/L，含白细胞3455.50（2288.68 - 4226.71）×10⁹/L，白细胞计数为13.79（10.21 - 20.72）×10⁹/L，含白细胞11.90（7.81 - 14.40）×10⁹/L。血红蛋白浓度为（3.28±1.25）g/L，含血红蛋白（2.62±1.17）g。血小板单采前后患者血小板计数分别为1263.00（1052.50 - 1807.50）×10⁹/L和（778.8±247.25）×10⁹/L（Z = 4.94，P＜0.01），白细胞计数分别为9.96（6.44 - 14.01）×10⁹/L和8.59（5.37，13.12）×10⁹/L（Z = 13.31，P＜0.05），血红蛋白浓度分别为（112.63±24.56）g/L和（109.55±24.46）g/L（t = 1.68，P＞0.05）。
采用连续流动离心及血液成分分离技术对血小板增多症患者进行血小板单采术，可明显降低过高的血小板比例，提高血小板单采效果。高容量血小板单采术可使白细胞计数有一定程度下降，但仍在正常范围内，且对血红蛋白水平无明显影响。
