利用 X 射线吸收和荧光光谱法对大豆叶片中锌的吸收和转运进行体内评价。
In Vivo Evaluation of Zn Foliar Uptake and Transport in Soybean Using X-ray Absorption and Fluorescence Spectroscopy.
机构信息
Centre for Nuclear Energy in Agriculture , University of São Paulo , Piracicaba , São Paulo 13416-000 , Brazil.
"Luiz de Queiroz" College of Agriculture , University of São Paulo , Piracicaba , São Paulo 13418-900 , Brazil.
出版信息
J Agric Food Chem. 2019 Nov 6;67(44):12172-12181. doi: 10.1021/acs.jafc.9b04977. Epub 2019 Oct 24.
Understanding the mechanisms of absorption and transport of foliar nutrition is a key step towards the development of advanced fertilization methods. This study employed X-ray fluorescence (XRF) and X-ray absorption near edge spectroscopy (XANES) to trace the in vivo absorption and transport of ZnO and ZnSO(aq) to soybean leaves (). XRF maps monitored over 48 h showed a shape change of the dried ZnSO(aq) droplet, indicating Zn absorption. Conversely, these maps did not show short movement of Zn from ZnO. XRF measurements on petioles of leaves that received Zn treatments clarified that the Zn absorption and transport in the form of ZnSO(aq) was faster that of ZnO. Solubility was the major factor driving ZnSO(aq) absorption. XANES speciation showed that in planta Zn is transported coordinated with organic acids. Because plants demand Zn during their entire lifecycle, the utilization of sources with different solubilities can increase Zn use efficiency.
了解叶面营养吸收和运输的机制是开发先进施肥方法的关键步骤。本研究采用 X 射线荧光（XRF）和 X 射线吸收近边光谱（XANES）技术追踪 ZnO 和 ZnSO₄（aq）在大豆叶片中的体内吸收和运输。48 小时监测的 XRF 图谱显示，干燥的 ZnSO₄（aq）液滴形状发生变化，表明 Zn 被吸收。相反，这些图谱没有显示 ZnO 中的 Zn 短距离移动。对接受 Zn 处理的叶片叶柄进行的 XRF 测量表明，以 ZnSO₄（aq）形式吸收和运输 Zn 的速度快于 ZnO。溶解度是驱动 ZnSO₄（aq）吸收的主要因素。XANES 形态分析表明，植物体内 Zn 的运输与有机酸配位。由于植物在整个生命周期中都需要 Zn，因此利用溶解度不同的来源可以提高 Zn 的利用效率。
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