[Effect of transfusing blood components containing on infection in BALB/c mice].

OBJECTIVE

To evaluate the effects of intravenous injection of different blood components containing on infection in mice.

METHODS

Healthy mice were infected with , and then blood samples were collected from the mouse orbit to prepare whole blood, serum-free blood components and pure red blood cells containing . Twenty seven BALB/c mice were divided into three groups, including the whole blood group, the serum-free blood component group and the pure red blood cell group, of 9 mice in each group, and then, each group was divided into three subgroups, of 3 mice in each subgroup, which were injected with 100 μL of blood components containing at concentrations of 9.00, 0.90, 0.09 parasites/μL (900, 90, 9 parasites) via the tail vein, respectively. Blood samples were collected from the mouse tail tip every other day since one day post-injection to prepare thin blood smears. Following Giemsa staining of blood smears, infection was identified in red blood cells using microscopy.

RESULTS

Following injection of 900 parasites, was identified in the peripheral blood in the whole blood group and the serum-free blood component group 3 days post-injection, and the density of parasites started to increase 15 days post-injection and peaked 21 days post-injection, with 2.21% and 1.76% rates of infection in red blood cells, respectively. Subsequently, the density of parasites declined, and the percentage of infection in red blood cells tended to be 0 31 days post-injection. During the study period, no was found in the peripheral blood in the pure red blood cell group. Following injection of 90 parasites, was identified in the peripheral blood in the whole blood group 3 days post-injection, and the density of parasites increased 15 days post-injection and peaked 21 days post-injection, with a 1.35% rate of infection in red blood cells, while the percentage of infection in red blood cells tended to be 0 31 days post-injection. During the study period, no was detected in the peripheral blood in the serum-free blood component group or the pure red blood cell group. Following injection of 9 parasites, no was detected in the peripheral blood in the whole blood group, the serum-free blood component group or the pure red blood cell group.

CONCLUSIONS

Blood components and dose of parasites may affect intravenous injection of injection in mice, and transfusion of blood components may case a risk of infection.