Rezapour-Firouzi Soheila, Kheradmand Fatemeh, Shahabi Sharam, Tehrani Ali Asghar, Mazloomi Ebrahim, Mohammadzadeh Adel
Cellular and Molecular Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, I.R. Iran.
Solid Tumor Research Center, Urmia University of Medical sciences, Urmia, I.R. Iran.
Res Pharm Sci. 2019 Mar 8;14(2):146-154. doi: 10.4103/1735-5362.253362. eCollection 2019 Apr.
T helper (Th)-17 mediate inflammation in both peripheral tissues and the central nervous system. Signal transducer and activator of transcription factor3 (STAT3) is required for Th-cell pathogenicity and its activation in the brain has been demonstrated during the acute phase of experimental autoimmune encephalomyelitis (EAE) through the mammalian target of rapamycin (mTOR) signaling. Rapamycin (RAPA), an inhibitor of mTOR, can drive Forkhead box P3 (FOXP3) induction as a regulatory factor. The aim of this study was to determine the effects of hemp seed/evening primrose oils (HSO/EPO) supplement on the expression of FOXP3, STAT3, and interleukin (IL)-17 genes in EAE lymph nodes. EAE was induced by myelin oligodendrocyte glycoprotein peptide in mice, and then the mice were assigned to three treatment groups compared to two control groups (EAE and naive). The histological findings of the spinal cord were evaluated. To determine the expression of FOXP3, STAT3, and IL-17 genes in the lymphocytes, qRT-PCR was used. Our results showed that EAE severity was reduced in HSO/EPO mice by reducing the expression of STAT3 and IL-17 genes and increasing the expression of FOXP3 gene, which was confirmed by slight inflammation in the spinal cord. Histological findings showed a significant improvement in the HSO/EPO group. Our findings suggest that the HSO/EPO treatment can be used to ameliorate the demyelination of spinal cord, which was confirmed by immunological and histological findings.
辅助性T细胞（Th）-17介导外周组织和中枢神经系统中的炎症。信号转导子和转录激活因子3（STAT3）是Th细胞致病性所必需的，并且在实验性自身免疫性脑脊髓炎（EAE）急性期，通过哺乳动物雷帕霉素靶蛋白（mTOR）信号传导已证实在大脑中其被激活。雷帕霉素（RAPA），一种mTOR抑制剂，可诱导作为调节因子的叉头框P3（FOXP3）。本研究的目的是确定大麻籽/月见草油（HSO/EPO）补充剂对EAE淋巴结中FOXP3、STAT3和白细胞介素（IL）-17基因表达的影响。用髓鞘少突胶质细胞糖蛋白肽在小鼠中诱导EAE，然后将小鼠与两个对照组（EAE组和未处理组）相比分为三个治疗组。评估脊髓的组织学发现。为了确定淋巴细胞中FOXP3、STAT3和IL-17基因的表达，采用qRT-PCR。我们的结果表明，HSO/EPO处理的小鼠中EAE严重程度降低，这是通过降低STAT3和IL-17基因的表达以及增加FOXP3基因的表达实现的，脊髓中的轻微炎症证实了这一点。组织学发现显示HSO/EPO组有显著改善。我们的研究结果表明，HSO/EPO处理可用于改善脊髓脱髓鞘，免疫和组织学发现证实了这一点。
